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2022, Vol. 26 ›› Issue (10): 1510-1514Construction of icariin/polycaprolactone nanofiber membrane and its osteogenic ability in vitroDong Zhiheng, Gao Yan, Liu Zhen, Han Xiaoqian
Abstract: BACKGROUND: In bone tissue engineering, the application of electrospinning makes bone growth factor play a long-term role. Icariin has been shown to promote the proliferation of MC3T3-E1 cells. OBJECTIVE: To construct icariin/polycaprolactone nanofiber membrane, and evaluate its biocompatibility and osteogenic ability in vitro. METHODS: Coaxial electrospinning technology was used to prepare icariin/polycaprolactone nanofiber membrane, and their morphology and drug release in vitro were characterized. MC3T3-E1 cells were cultured in vitro and divided into four groups. The blank group was treated with conventional culture. The nanofiber membrane group was treated with polycaprolactone nanofiber membrane. The drug group was treated with medium containing icariin. The experimental group was treated with icariin/polycaprolactone nanofiber membrane. Cytoskeleton morphology, proliferation, alkaline phosphatase activity, and osteocalcin mRNA expression were detected in each group. RESULTS AND CONCLUSION: (1) Scanning electron microscope revealed that the icariin-loaded/ polycaprolactone electrospinning nanofiber membrane showed a three-dimensional structure, uniform void size, aperture of 100-200 µm, distribution range of 300-600 nm. (2) The cumulative drug release of icariin/polycaprolactone nanofiber membrane observed within one month demonstrated that the drug release was about 45% in the first 1-5 days. The drug release was slow and sustained in the later period, and more than 80% in the 30 days. (3) After 24 hours of culture, laser confocal microscopy showed that the cells adhered to the nanofiber membrane, and the actin and internal structure of the cells were clear. (4) CCK-8 assay exhibited that the cell proliferation of the experimental group was faster than that of the other three groups at 4 and 7 days (P < 0.05). (5) Alkaline phosphatase activity was higher in the experimental group than that in the other three groups at 7, 14, and 21 days (P < 0.05). Osteocalcin mRNA expression was higher in the experimental group than that in the other three groups at 7 days after culture (P < 0.05). (6) The results show that electrospinning can be used to construct icariin/polycaprolactone nanofiber membrane, which has good drug release and bone promoting properties in vitro. Key words:bone, materials, polycaprolactone, nanofiber membrane, electrospun membrane, scaffold, icariin, osteoblast, proliferation |