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2022, Vol. 26 ›› Issue (11): 1648-1655Protective effect of astaxanthin on tert-butyl hydrogen peroxide-induced chondrocyte damageZhu Chunhui1, 2, Zhang Yi3, Song Huanghe2, Liang Wenwei2
Abstract: BACKGROUND: Cartilage damage is a common orthopedic disease. Studies on the mechanism of cartilage repair are important for the improvement of therapeutic strategies in clinical practice. Astaxanthin has a protective effect on cartilage damage; however, the related mechanism is not yet elucidated. OBJECTIVE: To study the protective effect of astaxanthin against tert-butyl hydrogen peroxide-induced damage to C28/I2 cells and to explore how circHP1BP3 is involved in this protect effect. METHODS: Tert-butyl hydrogen peroxide was used to induce damage to C28/I2 cells. The protective effect of astaxanthin was evaluated by cell viability, apoptosis, inflammation, oxidative stress, and degradation of extracellular matrix. RT-qPCR was used to explore the expression of circRNAs. The role of circHP1BP3 in the protective effect of astaxanthin was investigated by the use of circHP1BP3-overexpression plasmid or small-interference RNAs. RESULTS AND CONCLUSION: Tert-butyl hydrogen peroxide induced damage of C28/I2 cells by decreasing cell viability, and increasing cell apoptosis, inflammatory effect, oxidative stress, and degradation of the extracellular matrix. Astaxanthin protected C28/I2 cells from tBHP-induced cell damage. Tert-butyl hydrogen peroxide decreased the expression of circHP1BP3 in C28/I2 cells, while astaxanthin increased the expression of circHP1BP3 in tBHP-treated cells. circHP1BP3-overexpression recovered C28/I2 cells from tBHP-induced cell damage. circHP1BP3 silencing inhibited the protective effect of astaxanthin on tBHP-induced C28/I2 cell damage. To conclude, astaxanthin can protect C28/I2 chondrocytes from tBHP-induced cell damage via circHP1BP3. Key words:astaxanthin, circHP1BP3, chondrocyte, cell damage, circRNA, tert-butyl hydrogen peroxide, oxidative stress |