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2022, Vol. 26 ›› Issue (12): 1944-1949

Cell sheet technology applied in bone tissue engineering

Tan Xianyu, Liao Wenbo   

  1. Department of Orthopedics, the Second Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China

  • Received:2021-07-28 Revised:2021-07-30 Accepted:2021-08-24 Online:2022-04-28 Published:2021-12-15

  • Contact: Liao Wenbo, MD, Professor, Doctoral supervisor, Department of Orthopedics, the Second Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China

  • About author:Tan Xianyu, Master, Physician, Department of Orthopedics, the Second Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China

  • Supported by:

    the National Natural Science Foundation of China, No. 82060415 (to LWB)



Abstract: BACKGROUND: Cell sheet technology has broad application prospects in tissue regeneration and repair. What is more, it also has an important role and application value in the field of bone tissue engineering.
OBJECTIVE: To review the application and existing problems of cell sheet technology in bone tissue engineering.
METHODS: CNKI, PubMed, and Elsevier were searched for relevant articles regarding the application of cell sheet technology in bone tissue engineering published from January 2000 to July 2021. The key words were “cell sheet technology, cell sheet, bone, bone defect, bone repair, tissue engineering, bone tissue engineering” in Chinese and English, respectively. The article types were review, basic and clinical studies.

RESULTS AND CONCLUSION: Cell sheet technology is a new technology for obtaining seed cells, which not only acquires the desired seed cells, but also retains the abundant extracellular matrices and cell-cell connections. Therefore, it has broad application prospects in tissue regeneration and repair. The current technologies for cell sheet preparation mainly include: temperature response systems, mechanical systems, magnetic response systems, light response systems, and pH systems. Various technologies have their own advantages and disadvantages in the preparation of cell sheets. Among them, temperature response systems and magnetic response systems are currently widely used. There are many types of cell sheets, which can be divided into different types of cell sheets according to different cell sources. It can also be divided into single-layer cell sheet, double-layer cell sheet, and multi-layer cell sheet according to the number of sheet layers. In terms of bone tissue engineering, the cell sheet technology can be used alone or combined with various other scaffolds to enhance its mechanical strength in the repair of bone defects. In addition, adding growth factors or small molecular substances into cell sheets provides new ideas for the application of cell sheets.

Key words:cell sheet technology, tissue engineering, bone tissue engineering, scaffold material, seed cell, bone defect, bone repair, bone


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