2022, Vol. 26 ›› Issue (12): 1944-1949

RESULTS AND CONCLUSION: Cell sheet technology is a new technology for obtaining seed cells, which not only acquires the desired seed cells, but also retains the abundant extracellular matrices and cell-cell connections. Therefore, it has broad application prospects in tissue regeneration and repair. The current technologies for cell sheet preparation mainly include: temperature response systems, mechanical systems, magnetic response systems, light response systems, and pH systems. Various technologies have their own advantages and disadvantages in the preparation of cell sheets. Among them, temperature response systems and magnetic response systems are currently widely used. There are many types of cell sheets, which can be divided into different types of cell sheets according to different cell sources. It can also be divided into single-layer cell sheet, double-layer cell sheet, and multi-layer cell sheet according to the number of sheet layers. In terms of bone tissue engineering, the cell sheet technology can be used alone or combined with various other scaffolds to enhance its mechanical strength in the repair of bone defects. In addition, adding growth factors or small molecular substances into cell sheets provides new ideas for the application of cell sheets.

Key words:cell sheet technology, tissue engineering, bone tissue engineering, scaffold material, seed cell, bone defect, bone repair, bone