2022, Vol. 26 ›› Issue (13): 1974-1978

Histological comparison between goat bone marrow mesenchymal stem cells and adhesive fibrin for the repair of annulus fibrosus defect of intervertebral discs

Xu Xiang1, Wu Yimin1, Li Shuwen1, Ma Libo2, Wang Yupeng1, Yu Yingnan1, Sun Tao3, Zhang Yuan4, Ren Wei5, Yin Heping1

1Department of Minimally Invasive Spinal Surgery, 2Department of Nursing, 3Department of Emergency, Second Affiliated Hospital of Inner Mongolia Medical College, Huhhot 010030, Inner Mongolia Autonomous Region, China; 4Department of Surgical Anesthesiology, Inner Mongolia International Hospital, Huhhot 010030, Inner Mongolia Autonomous Region, China; 5Graduate College, Inner Mongolia Medical University, Huhhot 010059, Inner Mongolia Autonomous Region, China

Received:2021-05-12 Revised:2021-05-14 Accepted:2021-06-28 Online:2022-05-08 Published:2021-12-16
Contact: Yin Heping, Professor, Chief physician, Master’s supervisor, Department of Minimally Invasive Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical College, Huhhot 010030, Inner Mongolia Autonomous Region, China
About author:Xu Xiang, MD, Attending physician, Department of Minimally Invasive Spinal Surgery, Second Affiliated Hospital of Inner Mongolia Medical College, Huhhot 010030, Inner Mongolia Autonomous Region, China
Supported by:
Engineering Project on Science and Technology of Inner Mongolia Medical University, No. YKD2018KJBW(LH)006 (to XX); Natural Science Foundation of Inner Mongolia Autonomous Region, No. 2019BS03034 (to XX)

Abstract: BACKGROUND: At present, after lumbar discectomy, annulus fibrosus rupture still exists, easy to form recurrent lumbar disc herniation. How to repair the broken annulus fibrosus is a hot issue in tissue engineering.
OBJECTIVE: To compare the ability of goat bone marrow mesenchymal stem cell transplantation with gelatin sponge as carrier and adhesive fibrin repair for annulus fibrosus defects.
METHODS: Bone marrow mesenchymal stem cells were extracted and isolated from the goat iliac bone and transferred for culture. Fifteen goats were randomized into control, adhesion, and transplant groups (n=5 per group and 30 discs per group). The 0.75 cm×0.75 cm disc annulus fibrosus defect was made with surgical instruments. In the binding group, the damage was bonded with fibrin adhesive. In the transplant group, the gelatin sponge (0.75 cm×0.75 cm) with bone marrow mesenchymal stem cell suspension (cell concentration: 5×109/L, 10 μL) was placed into the annulus fibrosus defect and the lesion was stitched layer by layer. At 6 and 12 weeks after surgery, annulus fibrosus tissue was removed for hematoxylin-eosin staining, Masson staining, AB-PAS staining, and type II collagen staining.

RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that the number of chondrocytes, collagen cells and bone cells in the transplant group increased, the cells were mature and the repair ability was stronger compared with the other two groups. Masson staining demonstrated that the number of fibroblasts and fibrous tissue was more in the transplant group compared with the other two groups. AB-PAS staining exhibited that more chondrocytes in the transplant group were involved in repair compared with the other two groups. Type II collagen staining showed that the content of type II collagen was highest in the transplant group. The above results confirm that goat bone marrow mesenchymal stem cells have a perfect ability to repair annulus fibrosus defects.

Key words:bone marrow mesenchymal stem cells, adhesive fibrin, histological observation, annulus fibrosus, type II collagen, Masson staining, AB-PAS staining, hematoxylin-eosin staining

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