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2022, Vol. 26 ›› Issue (13): 2040-2044Biocompatibility of plant-derived nerve conduits to nasal ectomesenchymal stem cellsZheng Youfei1, 2, Shi Wentao2, Liu Xiaogu2, Zhuang Qin2, Lü Demin1, 2, Zhang Jiayin2, Yuan Yiwen2, Zhang Zhijian2, Xu Xiaofeng1, 2
Abstract: BACKGROUND: There are many reports on the repair of peripheral nerve injury by nerve conduit bridging nerve gap instead of autogenous nerve graft. The nasal ectomesenchymal stem cell is an important seed cell for the transplantation of peripheral nerve injury. OBJECTIVE: To investigate the biocompatibility of nasal ectomesenchymal stem cells and plant-derived neural conduits and the spontaneous differentiation and growth of nasal ectomesenchymal stem cells on the surface of the conduit. METHODS: Tube culms with an inner diameter of 0.2 cm were selected in nature. After physical and chemical removal of pulp, their fibrous tubular shape was retained. The extracellular matrix protein laminin was modified by genipin. Nasal ectomesenchymal stem cells were isolated and cultured in vitro, and divided into three groups: the blank group (blank culture plate), the unmodified group (unmodified conduit) and the modified group (modified conduit). MTT assay was performed at 1, 3, 5, 7, and 14 days to evaluate cell viability. Biocompatibility was assessed in vitro. The effect of the conduit on the spontaneous differentiation of nasal mucosa-derived ectodermal mesenchymal stem cell was observed by immunoblotting and immunofluorescence two weeks after cell implantation and culture. RESULTS AND CONCLUSION: (1) Nasal ectomesenchymal stem cells grow well and were oriented on the surface of plant duct fibers, and plant conduits were biocompatible with nasal ectomesenchymal stem cells. (2) The expression levels of MBP and S100 in nasal ectomesenchymal stem cells implanted on the surface of the catheter were higher than those in the blank and unmodified groups. (3) It is concluded that the differentiation of nasal ectomesenchymal stem cells into Schwann cells can be promoted by laminin-modified plant-derived nerve conduits, which may provide a new theoretical basis for the transplantation and repair of peripheral nerve cells loaded with natural plant-derived nerve conduits. Key words:stem cells, transplantation, nasal mucosa, ectodermal mesenchymal stem cell, fiber plants, nerve conduit, biocompatibility, peripheral nerve injury |