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2022, Vol. 26 ›› Issue (13): 2045-2049Effect of brain-derived neurotrophic factor modified human amniotic mesenchymal stem cell transplantation on cognitive function in Alzheimer’s disease ratsWang Yuxiang, Cui Chuanju, Li Yanling, Li Aifan
Abstract: BACKGROUND: Studies have found that human mesenchymal stem cells modified by brain-derived neurotrophic factor (BDNF) can be used to treat spinal cord injury and traumatic brain injury, but whether there is a curative effect on Alzheimer’s disease is rare in the past reports. OBJECTIVE: To observe the effects of BDNF modified human amniotic mesenchymal stem cells (hAMSCs) on learning and memory ability and expression of choline acetyltransferase in hippocampus and nerve growth factor in basal forebrain of rats with Alzheimer’s disease. METHODS: Forty healthy male adult SD rats were randomly divided into control group, model group, hAMSCs group and BDNF-hAMSCs group with 10 rats in each group. Bilateral hippocampal injection of β-amyloid 25-35 was used to construct an Alzheimer’s disease model. On day 14, 10 μL of hAMSCs or 10 μL of BDNF were injected into the posterior ventricle to transfect hAMSCs. At 2 weeks after transplantation, learning and memory abilities of rats were evaluated by Morris water maze test. Choline acetyltransferase expression in hippocampus was detected by immunohistochemistry. Nerve growth factor expression in the basal forebrain was detected by RT-PCR. RESULTS AND CONCLUSION: (1) At 3, 4, and 5 days of Morris water maze test, the escape latencies of the hAMSCs group and the BDNF-hAMSCs group were lower than that in the model group (P < 0.05). At 4 and 5 days, the escape latency of the BDNF-hAMSCs group was lower than that of the hAMSCs group (P < 0.05). (2) The number of choline acetyltransferase positive neurons and nerve growth factor expression in the basal forebrain in hippocampus of model group were significantly lower than those of control group (P < 0.05). The number of choline acetyltransferase positive neurons in the hippocampus and nerve growth factor expression in the basal forebrain of the hAMSCs group and the BDNF-hAMSCs group were higher than those of the model group (P < 0.05), and the number of choline acetyltransferase positive neurons and nerve growth factor expression in the BDNF-hAMSCs group were higher than those in the hAMSCs group (P < 0.05). (3) Results suggest that modification of hAMSCs with BDNF can further improve the efficacy of stem cells in the treatment of Alzheimer’s disease, and can significantly increase the expression levels of choline acetyltransferase in the hippocampus and nerve growth factor in the basal forebrain. Key words:stem cells, human amniotic mesenchymal stem cells, brain-derived neurotrophic factor, alzheimer’s disease, choline acetyltransferase, brain nerve growth factor, cognitive function |