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2022, Vol. 26 ›› Issue (13): 2050-2055Basic fibroblast growth factor promotes the proliferation of stem cells from the apical papilla through activation of Wnt/beta-Catenin signaling pathwayFu Yi1, Zhang Yu1, He Mei2, Che Yanlin3, Liang Jing2, Wu Jiayuan2
Abstract: BACKGROUND: When basic fibroblast factors act on fibroblasts, they can induce their proliferation by activating Wnt signaling. However, in the study of its effect on apical dental papilla stem cells, whether it can activate the Wnt/β-Catenin signaling pathway and the effect of activating this pathway on the biological activities of apical dental papilla stem cells remains unclear. OBJECTIVE: To investigate the regulatory role of basic fibroblast growth factor on the proliferation of stem cells from apical papilla. METHODS: Human stem cells from apical papilla were cloned and cultured through enzymatic digestion and limiting dilution assay, and then treated with 15 μg/L basic fibroblast growth factor for 2 days. RT-PCR and western blot assay were used to detect the expression of β-Catenin, TCF1, TCF3, and LEF1 mRNAs and proteins. The effect of basic fibroblast growth factor on the proliferation of stem cells from apical papilla was further studied in the case of Wnt/β-Catenin signaling pathway inhibition. The basic fibroblast growth factor (15 μg/L) + Dkk-1 group, basic fibroblast growth factor (15 μg/L) group, and negative control group were set. The proliferation of stem cells from apical papilla in each group was compared by MTT assay and EdU assay. RESULTS AND CONCLUSION: (1) The expression levels of β-Catenin, TCF1, TCF3, and LEF1 mRNAs and proteins were upregulated by 15 μg/L basic fibroblast growth factor on stem cells from apical papilla (P < 0.05). (2) The stem cells from apical papilla of the basic fibroblast growth factor (15 μg/L) group showed stronger proliferation activity than that in the negative control group and basic fibroblast growth factor (15 μg/L) + Dkk-1 group (P < 0.05). (3) Results suggest that basic fibroblast growth factor can promote the expression of key molecules β-Catenin in Wnt/β-Catenin signaling pathway and downstream molecules TCF1, TCF3, and LEF1. Wnt/β-Catenin signaling pathway participates in the regulation of basic fibroblast growth factor on the proliferation of stem cells from apical papilla. Key words:stem cells, stem cells from apical papilla, basic fibroblast growth factor, Wnt/β-Catenin, signaling pathway, proliferation |