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2022, Vol. 26 ›› Issue (13): 2127-2132Regulatory effects of hypoxic culture on physiological activities of mesenchymal stem cellsLiu Ziwen, Wang Wenbo
Abstract: BACKGROUND: Mesenchymal stem cells are multipotent stem cells owing the ability of self-renewal and multiple differentiation potential, which promise mesenchymal stem cells to differentiate into several cell types, including osteoblasts, adipocytes, chondrocytes, myocytes and neurons and so on. Mesenchymal stem cells can secrete factors associated with cell proliferation and survival, immunomodulation, and recruitment. These potentials and characteristics make mesenchymal stem cells an ideal cell source for cell-based treatment of tissues repair in regenerative medicine. Since the oxygen constitution only at range of 3%-8% in healthy bone marrow which is much lower than the normal oxygen concentration of 21%, and this concentration will become lower in the injured tissue microenvironment. Thus, the fate of mesenchymal stem cells in hypoxic environment attracts lots of attention. OBJECTIVE: To explicate the roles of hypoxic condition in mesenchymal stem cells properties and the therapeutic effect of mesenchymal stem cells transplantation. METHODS: The articles about effect of hypoxia on mesenchymal stem cell were searched in CNKI, Wanfang, and PubMed from 2006 to 2020. The search key words were “mesenchymal stem cells, hypoxia culture, stem cell therapy” in Chinese, and “mesenchymal stem cells, hypoxia, regenerative therapy” in English. The collected literature was sorted out to eliminate obsolete concepts and repeated views, and 73 articles were finally selected for analysis. RESULTS AND CONCLUSION: Hypoxic culture could promote cell proliferation, migration ability of mesenchymal stem cells and regulate cell differentiation. In clinical treatment, hypoxic preconditioning could regulate the differentiation ability of mesenchymal stem cells, improve cell survival rate, accelerate tissue healing, and improve the therapeutic effect. Hypoxic culture on the regulation of mesenchymal stem cells provides a new idea for the clinical treatment of osteoporosis and transplantation regenerative therapy. Key words: stem cells, mesenchymal stem cells, hypoxia, proliferation, differentiation, repair, review |