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2022, Vol. 26 ›› Issue (14): 2155-2160Effect of panax notoginseng saponins on platelet-rich plasma promoting bone defect healing in rabbitsLi Shijie, Ma Liqiong, Xiong Xianmei, Zhang Yan, Chen Zijie, Feng Junming, Gao Yijia, Zeng Zhanpeng
Abstract: BACKGROUND: Panax notoginseng saponins combined with platelet-rich plasma can up-regulate the expression of factors related to osteogenesis and vascularization, and promote bone healing. OBJECTIVE: To observe the release of growth factors from platelet-rich plasma after intragastric administration of panax notoginseng saponin and its effects on bone defect healing in rabbits. METHODS: Eighteen New Zealand white rabbits were randomly divided into three groups: PNS group, control group and blank group. PNS group was treated with panax notoginseng saponins by gavage for 2 weeks. Control group was given 10 mL of normal saline by gavage for 2 weeks. Blank group were fed normally. Platelet-rich plasma was obtained by centrifugation in the PNS group together with the control group. After activation, growth factor release was detected by ELISA at different time points (0, 2 hours, 1, 3, 5, and 7 days after activation). One week later, the model of radial bone defect was established in each rabbit. PNS and control groups were implanted with autologous platelet-rich plasma. The bone defect healing of the three groups was compared based on the results from X-ray examination, gross observation, and hematoxylin-eosin staining. The study protocol was approved by the Experimental Animal Ethics Committee of the First Affiliated Hospital of Guangzhou University of Chinese Medicine (approval No. TCMF1-2019062). RESULTS AND CONCLUSION: The release of growth factors: In the PNS group, the release concentrations of vascular endothelial growth factor A on the 7th day, transforming growth factor-β on the 1st day, basic fibroblast growth factor on the 3rd and 5th days after activation were higher than those of the control group (P < 0.01). Bone defect healing: Two months after operation, X-ray examination results showed that better bone healing was observed in the PNS group than the control group, and bone healing in these two groups was significantly better than that in the blank group. Three months after operation, bone defects were healed in all the three groups. Hematoxylin-eosin staining results showed that the trabecular density was higher in the PNS group than the control group, and was worst in the blank group. All these findings indicate that the intervention of Panax notoginseng saponins can up-regulate the concentration of growth factors released from platelet-rich plasma, which has more advantages in promoting the healing of rabbit bone defects than the use of platelet-rich plasma alone. Key words:bone defect, platelet-rich plasma, panax notoginseng saponins, growth factor, rabbit radius, bone healing, bone defect repair |