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2022, Vol. 26 ›› Issue (14): 2207-2213Platelet-rich fibrin combined with induced bone matrix repairs bone defects around oral implants in rabbitsGao Xixin1, 2, 3, Wang Xi1, Fan Xuhui4, Cui Yi4, Yang Wei4, Zhao Yunzhuan4
Abstract: BACKGROUND: Platelet-rich fibrin and artificial bone materials have been widely used in the repair and regeneration of peri-implant bone defects. However, there was a lack of research on the osteogenesis of the two materials in different proportions. OBJECTIVE: To investigate the effect of platelet-rich fibrin combined with bone matrix on the regeneration of peri-implant bone defects in rabbits. METHODS: A bone defect model around the oral implant was established in 12 large-ear white rabbits. Three cylindrical bone defect areas with a diameter of 5 mm and a depth of 2 mm were prepared along the lower edge of the right mandible, which were numbered 1-3 from the mesial to the distal. After placement of titanium screws, the 2:1, 1:1, and 1:2 mixtures of platelet-rich fibrin and bone matrix were used respectively to fill in the defects. Another three same bone defects were prepared along the lower edge of the left mandible, which were numbered 4-6 from the distal to the mesial. Titanium screw screws were placed, No. 4 defect was filled with nothing as a blank control, No. 5 was filled with platelet-rich fibrin, and No. 6 was filled with bone matrix. Twelve rabbits models were randomized into 4-, 8-, 12-week groups, with 4 rabbits in each group. One rabbit from each group was randomly selected, killed and fluorescently labeled. Tetracycline (25 mg/kg) and calcein (5 mg/kg) were subcutaneously administered at 10 and 3 days before death, respectively. Other animals were killed at 4, 8, and 12 weeks after modeling, and the osteogenic effect was statically analyzed by general observation, hematoxylin-eosin staining, fluorescent labeling, and methylene blue/acid fuchsin staining. RESULTS AND CONCLUSION: Bone formation rate from high to low was: platelet rich fibrin/bone matrix 2:1 > platelet rich fibrin > platelet rich fibrin/bone matrix 1:1 > platelet rich fibrin/bone matrix 1:2 > bone matrix > blank control. Both platelet-rich fibrin and bone matrix have a good effect in bone repairing bone defect. When platelet rich fibrin and medical induced bone matrix are used in combination, the osteogenic effect becomes better with the increase of platelet-rich fibrin concentration. The combined application of platelet-rich fibrin and bone matrix can accelerate bone healing of implants. Key words:platelet-richfibrin, bone matrix, bone defect, implant, rabbit, modeling |