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2022, Vol. 26 ›› Issue (17): 2667-2672Constructing a mouse model of periodontitis with occlusal traumaYang Qin, Wang Min
Abstract: BACKGROUND: Although it is essential to establish a model of periodontitis with occlusal trauma for exploring the role of occlusal trauma in periodontitis, there is yet no stable and reliable method to establish an animal model of periodontitis with occlusal trauma. OBJECTIVE: To establish a stable, reliable and efficient animal model of periodontitis with occlusal trauma. METHODS: Animal models of occlusal trauma, periodontitis, and periodontitis with occlusal trauma were made in BALB/c mice by composite resin bonding, oral implantation of Porphyromonas gingivalis, and their combination. Healthy mice acted as control group. Micro-CT scan was used to detect alveolar bone resorption and fluorescent quantitative PCR was used to detect the expression of tumor necrosis factor α, interleukin 1β, and interleukin 6 in the alveolar bone, in order to determine whether the animal model of periodontitis with occlusal trauma was successfully established. RESULTS AND CONCLUSION: Compared with the control group, there was no obvious alveolar bone resorption in the simple occlusal trauma group, while the alveolar bone resorption was aggravated in the simple periodontitis group and the periodontitis with occlusal trauma group, especially in the latter group. Compared with the control group, the relative mRNA expression of tumor necrosis factor α and interleukin 1β was significantly increased in the simple occlusal trauma group (P < 0.01). Compared with control group, the expression levels of tumor necrosis factor α, interleukin 1β, and interleukin 6 mRNA were significantly up-regulated in the simple periodontitis group and the periodontitis with occlusal trauma group, and moreover, these expression levels were higher in the periodontitis with occlusal trauma group than the simple periodontitis group (P < 0.01). All these suggest that the animal model of periodontitis with occlusal trauma is successfully established. Key words:occlusal trauma, periodontitis, animal model, bone resorption, inflammation, alveolar bone, mouse |