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2022, Vol. 26 ›› Issue (17): 2690-2695Effects of aerobic exercises on JAK2/STAT5 signal pathway in the liver of mice with non-alcoholic fatty liver diseaseZhang Shuling, Li Junhan, Wang Jiaqian, Li Yalong, Wang Chun
Abstract: BACKGROUND: The Janus kinase 2/signal transducer and activator of transcription 5 (JAK2/STAT5) signaling pathway plays an important role in lipid metabolism. The effective prevention and treatment of non-alcoholic fatty liver by exercise is closely related to the improvement of visceral lipid deposition and lipid metabolism. OBJECTIVE: To explore the effect of aerobic exercise on the JAK2/STAT5 signaling pathway in the liver of non-alcoholic fatty liver mice and its possible mechanism. METHODS: Forty-eight C57BL/6 male mice aged 6 weeks were randomly divided into a normal diet group (n=24) and a high-fat diet induction group (n=24). On the 10th weekend, four mice were randomly selected from each group for oil red O staining to observe the pathological morphological changes of the liver. After successful modeling, the normal diet group was randomly divided into a normal diet+quiet group (n=10) and a normal diet+exercise group (n=10); the high-fat diet induction group was randomly divided into a non-alcoholic fatty liver+quiet group (n=10) and a non-alcoholic fatty liver+exercise group (n=10), followed by 8 weeks of intervention until the end of the experiment. Liver pathological changes were observed, and prolactin receptor, JAK2, and phosphorylation of JAK2 and STAT5 in the liver of mice were detected. RESULTS AND CONCLUSION: Compared with the normal diet+quiet group, hepatic steatosis was increased and the protein expression of JAK2/STAT5 signaling pathway in the liver was decreased in the non-alcoholic fatty liver+quiet group. Compared with the non-alcoholic fatty liver+quiet group, hepatic steatosis was decreased and the protein expression of JAK2/STAT5 signaling pathway in the liver was increased in the non-alcoholic fatty liver+exercise group. The liver pathological indicators were significantly negatively correlated with prolactin receptor, JAK2, and phosphorylation of JAK2/STAT5 (P < 0.05). To conclude, aerobic exercise intervention can improve liver lipid deposition and the degree of hepatic steatosis by regulating the JAK2/STAT5 signaling pathway in the liver of mice with non-alcoholic fatty liver. Key words:aerobic exercise, non-alcoholic fat liver disease, Janus kinase 2/Signal Transducer and Activator of Transcription 5 signaling pathway, phosphorylation |