|
|
2022, Vol. 26 ›› Issue (17): 2713-2719Establishment and evaluation of neurogenic bladder model in rats with type 2 diabetes mellitusHan Xuke1, 2, Chen Yiding1, 2, Chen Huizhen1, 2, Yang Xiaomei1. 2, Hong Peipei1, 2, Chen Qiu1, 2
Abstract: BACKGROUND: The incidence of neurogenic bladder in type 2 diabetes is increasing. However, its pathogenesis is not clear and there is no effective treatment method. It is of great significance to construct an efficient and stable animal model for the study on its pathogenesis and treatment methods. OBJECTIVE: To establish a rat model of neurogenic bladder in type 2 diabetes mellitus. METHODS: Male Sprague-Dawley rats were divided randomly into control (n=10) and model (n=45) groups. Control group was given 8-week normal diet, and model group was given 8-week high fat diet. At the 4th week of high-fat diet, the model rats were randomly divided into three groups (n=15 per group) followed by single intraperitoneal injection of 20, 25, and 50 mg/kg streptozotocin respectively. The control group was treated with the equal amount of sodium citrate buffer. The general condition was observed, and the body mass and blood glucose level were recorded at 0, 4, and 8 weeks. At the 8th week, urodynamic parameters and bladder wet weight were detected, and histological changes of the bladder were observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION: High-fat diet combined with single intraperitoneal injection of streptozotocin (35 mg/kg) had higher survival rate and successful modeling rate (80%). Compared with the control group, the bladder capacity and residual urine volume increased, the maximum bladder pressure decreased, the wet weight and compliance of the bladder increased after single intraperitoneal injection of 35 mg/kg streptozotocin (P ≤ 0.05). In conclusion, 8-week high-fat diet combined with single intraperitoneal injection of 35 mg/kg streptozotocin is the best method to establish a rat model of type 2 diabetes mellitus with neurogenic bladder. Key words:streptozotocin, type 2 diabetes mellitus, neurogenic bladder, urodynamics, high-fat diet |