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2022, Vol. 26 ›› Issue (17): 2732-2737Effects of nuclear factor of activated T cells 5 on migration, invasion and cell cycle of human gastric cancer MGC803 cellsGuo Junfu, Gao Xia, Chang Jiarong, Gao Shiqi, Miao Lanying
Abstract: BACKGROUND: Recent studies have shown that high expression of nuclear factor 5 of activated T cells (NFAT5) is closely related to the occurrence and progression of a variety of tumors. Previous studies have found that NFAT5 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC803 cells. OBJECTIVE: To investigate the effects of NFAT5 on migration, invasion and cell cycle of human gastric cancer MGC803 cells. METHODS: There were two groups: NC-siRNA and NFAT5-siRNA. The siRNAs were transfected to silence NFAT5 gene expression in MGC803 cells. Cell scratch assay, Transwell assay and flow cytometry were performed to test migration ability, invasion ability, and cell cycle distribution, respectively. RESULTS AND CONCLUSION: The wound healing capacity of NC-siRNA group was significantly higher than that in the NFAT5-siRNA group. The number of cells passed through the ventricle in the NFAT5-siRNA group was obviously less than that in the NC-siRNA group [(134.63±25.62) vs. (195.00±60.41), P < 0.05]. Compared with the NC-siRNA group, the proportion of G1-phase cells was significantly increased [(60.03±0.55)% vs. (62.46±0.73)%, P < 0.05] and the proportion of S-phase cells was significantly decreased [(28.24±1.16)% vs. (25.44±1.15)%, P < 0.05]. Therefore, NFAT5 silencing in human gastric cancer MGC803 cells can inhibit cell migration and invasion abilities in vitro and change the cell cycle distribution, thereby reducing cell proliferation ability. NFAT5 is expected to be a new diagnostic and therapeutic target for gastric cancer. Key words:NFAT5, gastric cancer, cell, migration, invasion, cell cycle |