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2022, Vol. 26 ›› Issue (19): 3018-3023Effects of allogeneic bone marrow mesenchymal stem cells infusion on the expression of CXCL12/CXCR4 in the kidney of MRL/lpr lupus miceCheng Mengwei, Tang Yu, Ma Cui, Shi Wei, Zheng Wenjuan, Qiu Yingying, Rui Jinbing, Wang Yanru
Abstract: BACKGROUND: Chemokine CXCL12 and its receptor CXCR4 play a certain role in the pathogenesis of lupus nephritis, and it is of significance to explore the effect of bone marrow mesenchymal stem cells infusion on the expression of CXCL12/CXCR4 in lupus kidney for further study of the pathogenesis of lupus nephritis and the mechanism of bone marrow mesenchymal stem cells in the treatment of lupus nephritis. OBJECTIVE: To investigate the effect of allogeneic bone marrow mesenchymal stem cell infusion on the expression of CXCL12/CXCR4 in the kidney of MRL/lpr lupus mice. METHODS: Bone marrow mesenchymal stem cells of C57BL/6 mice were isolated and cultured, expanded to the third passage, and the cell concentration was 1×109 L-1. Totally 15 female MRL/lpr mice aged four to five weeks were selected and divided into MRL/lpr group (no tail vein infusion), PBS group (PBS injection at the end of 12 weeks), and bone marrow mesenchymal stem cell group (injection of bone marrow mesenchymal stem cells at the end of 12 weeks) (n=5 per group). An additional five C57BL/6 mice were selected as normal controls. Mice in each group were sacrificed at the end of 20 weeks of age. The expression of CXCL12 in peripheral blood plasma was detected by ELISA. The expression of CXCR4 on B lymphocyte surface in peripheral blood and kidney was analyzed by flow cytometry. The expression of CXCL12 in the kidney was demonstrated by immunohistochemistry. The pathological changes of the kidney tissue were observed by hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) Compared with the C57BL/6 group, the expression of CXCL12 in peripheral blood plasma and kidney was increased, and the expression of CXCR4 on B lymphocyte surface in peripheral blood and kidney was also increased in the MRL/lpr group. (2) Compared with PBS group, the expression levels of CXCL12 in peripheral blood plasma and kidney of lupus mice were decreased, and CXCR4 expression levels on the surface of B lymphocytes in peripheral blood and kidney of lupus mice were similarly decreased in the bone marrow mesenchymal stem cell group. (3) Hematoxylin-eosin staining suggested that kidney injury was relieved in lupus mice after infusion of bone marrow mesenchymal stem cells. (4) It is concluded that there is abnormal expression of CXCL12/CXCR4 in the kidney of MRL/lpr lupus mice, and bone marrow mesenchymal stem cell infusion may play a therapeutic role by affecting the expression of CXCL12/CXCR4. Key words:stem cells, bone marrow mesenchymal stem cells, lupus nephritis, systemic lupus erythematosus, chemokine, CXCL12, CXCR4 |