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2022, Vol. 26 ›› Issue (19): 3030-3035Effects of miR-7-5p on stem cell-like properties of human gastric cancer SGC-7901 cellsGuo Junfu, Li Xiangnan, Ma Tianchi, Miao Lanying
Abstract: BACKGROUND: The expression of miR-7 is down-regulated in various tumor tissues, including gastric cancer, and plays a major role in tumor suppression. It has been confirmed that miR-7 can affect a variety of cell biological functions, such as proliferation, apoptosis, migration, and invasion of gastric cancer cells. However, few studies have been conducted on the relationship between miR-7 and stem cell-like properties of gastric cancer cells, and the mechanism remains unclear. OBJECTIVE: To investigate the effects of miR-7-5p on stem cell-like properties of human SGC-7901 gastric cancer cells. METHODS: The gastric cancer cell line SGC-7901 was used as cell model. NC, miR-7-5p mimics, inhibitor NC and miR-7-5p inhibitor were transfected into cells, separately. The inverted fluorescent microscope was used to observe transfection efficiency at 48 hours post-transfection. The expression levels of miR-7-5p, Nanog and Sox2 mRNA were detected by RT-qPCR. Spheroid and soft-agar colony formation assays were performed to observe the abilities of spheroid and soft-agar colony formation, respectively. The proportion of CD24+CD44+ cell subsets was determined by flow cytometry. RESULTS AND CONCLUSION: (1) The expression of miR-7-5p was significantly increased or decreased in SGC7901 cells after transfection of miR-7-5p inhibitor or mimics compared with inhibitor NC or NC group (P < 0.01). (2) The numbers of spheroid and soft-agar colony formation of SGC-7901 cells were decreased in the miR-7-5p mimics group than those in NC group (P < 0.05, P < 0.01), but increased in miR-7-5p inhibitor group than those in inhibitor NC group (P < 0.05, P < 0.01). (3) The proportion of CD24+CD44+ cells in the miR-7-5p mimics group was significantly lower than that in the NC group (P < 0.01). The proportion of CD24+CD44+ cells in miR-7-5p inhibitor group was significantly higher than those in inhibitor NC group (P < 0.01). (4) The mRNA expression levels of Nanog and Sox2 in the miR-7-5p mimics group were significantly lower than those in the NC group, and the mRNA expression levels of Nanog and Sox2 in the miR-7-5p inhibitor group were significantly higher than those in the inhibitor NC group (P < 0.05, P < 0.01). (5) miR-7-5p can inhibit spheroid and soft-agar colony formation abilities of gastric cancer cells, and reduce the proportion of CD24+CD44+ cell subsets in gastric cancer cells. The potential mechanism may be related to the regulation of the expression of stemness genes Nanog and Sox2. Key words:stem cell-like property, gastric cancer, miR-7-5p, CD24, CD44, Nanog, Sox2 |