2022, Vol. 26 ›› Issue (2): 211-217
Asperosaponin VI therapy for Achilles tendinopathy in rabbits
In recent years, tendonopathy occurs frequently as a chronic orthopedic injury, and there is still a lack of effective treatment as yet. Dipsacus L. has the effect of tonifying the liver and kidney, strengthening bones, and sustaining broken injuries.
To analyze the repairing effect of Asperosaponin VI in a rabbit model of Achilles tendinopathy.
Forty-eight rabbits were randomized into a normal control group (n=8), a model group (n=32), and a noral saline group (n=8). In the model group, the model of tendinopathy was made at the left calcaneus attachment injected with prostaglandin E2, and the models were then randomized into four subgroups, which were treated with 0, 10, 20, and 40 mg/kg Asperosaponin VI, respectively. Treatments in each group were conducted once a day for 4 consecutive weeks. The study protocol was approved by the Animal Ethics Committee of Chengdu Sport University with an approval No. 21 on November 4, 2019.
Compared with the normal control group, the expression of matrix metalloproteinase-1 was significantly higher in the model group (P < 0.05). Compared with the model group, the expression of matrix metalloproteinase-1 was significantly reduced in the 20 and 40 mg/kg groups (P < 0.05). The expression of tissue inhibitor of matrix metalloproteinases 1 was significantly higher in the model group than the normal control group (P < 0.05), while the expression of tissue inhibitor of matrix metalloproteinases 1 was significantly reduced in the 10, 20 and 40 mg/kg groups compared with the model group (P < 0.05). The expression of transforming growth factor-β1 was significantly higher in the 10, 20 and 40 mg/kg groups compared with the model group (P < 0.05). The expression of plasminogen activator inhibitor 1 was significantly higher in the 40 mg/kg group than the model group (P < 0.05). Tendon fibers in the 40 mg/kg group were intact and arranged orderly, which were similar to normal tendon tissue. Therefore, Asperosaponin VI can inhibit vicious cycle of degeneration of collagen in the extracellular matrix, induce the proliferation of tenocytes and promote the healing. It may be a potential drug for the treatment of tendinopathy.
Asperosaponin VI, tendinopathy, healing, collagen, extracellular matrix, tenocyte, proliferation, fibrosis