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2022, Vol. 26 ›› Issue (2): 218-224

Pathological changes in rats with ischemic stroke induced by improved photochemical embolization

Li Shulun1, Hao Peng1, Hao Fei2, Duan Hongmei1, Zhao Wen1, Gao Yudan1, Yang Chaoyang1, Li Xiaoguang1   

  1. 1Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; 2Advanced Biomedical Engineering Innovation Center, Medicine & Engineering Innovation Institute, Beihang University, Beijing 100191, China

  • Received:2021-01-21 Revised:2021-01-22 Accepted:2021-02-05 Online:2022-01-18 Published:2021-10-27

  • Contact: Li Xiaoguang, MD, Professor, Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

  • About author:Li Shulun, Master, Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

  • Supported by:

    the National Natural Science Foundation of China, No. 81941011, 31730030, 31650001, 31320103903 (all to LXG), 31670988, 31971279 (both to YCY), 31771053 (to DHM), 31900749 (to HP); the National Key Research and Development Program of China, No. 2017YFC1104002 (to YCY) and 2017YFC1104001 (to LXG); Beijing Municipal Science and Technology Plan, No. Z181100001818007 (to YCY); 2018 Science and Technology Plan Key Project of Beijing Municipal Education Commission, No. KZ201810025030 (to YCY); Beijing Natural Science Foundation (Youth Projects), No. 7194243 (to HP) and 7214301 (to HF)



Abstract: BACKGROUND: A rat model of photochemical embolization can simulate ischemic stroke well, with the advantages of specific damage to the brain area, high reproducibility and low mortality. However, the choice of light source and the concentration of rose bengal injection have certain effects on the model. So, a detailed evaluation is needed to determine whether the animal model is established successfully.
OBJECTIVE: To establish a stable model of ischemic stroke in rats that is less liable to recover spontaneously, and to explore the pathological changes in the infarct region and behavior changes in rats.
METHODS: A total of 52 male Wistar rats were randomly divided into sham operation group (n=6) and photochemical embolization group (n=46). In the photochemical embolization group, 20, 40, and 80 mg/kg rose bengal was injected into the rat femoral vein to make photochemical embolization models, and meanwhile the optimal rose bengal concentration was screened out. The remaining 28 rats were subjected to photochemical embolization. Rats in the sham operation group were not irradiated with laser and were not injected with rose bengal dye. TTC staining was used to reveal the changes in infarct size at 1 day after injection with different concentrations of rose bengal. NeuN staining was used to observe neuronal death in the infarct region at 1, 3, 7, and 14 days after photochemical embolization. Iba-1, GFAP, and GLUT-1 staining were employed to observe the changes of inflammatory responses, glial scars and blood vessels in the infarct region after photochemical embolization. Cylinder experiment and grid error experiment were used to evaluate the behavioral changes of rats after photochemical embolization.

RESULTS AND CONCLUSION: Injection with 80 mg/kg rose bengal caused the largest size of stroke cavity, resulting in a high reproducibility and a low animal mortality. A stable stroke cavity could be formed within 7 days after injection. Relatively stable glial scar zone formed at 7 days after photochemical embolization, but inflammatory reactions gradually aggravated in 1-14 days. Photochemical embolization reduced the area of blood vessels near the stroke cavity, and the area tended to be stable at 14 days. The rats experienced long-term decline in sensation and motor function after photochemical embolization. To conclude, the rat model of photochemical embolization is stable and not easy to recover spontaneously, which is suitable for studying the pathological changes after ischemic stroke.

Key words:ischemic stroke, photochemical embolization, pathology, behavior, rat, animal model


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