2022, Vol. 26 ›› Issue (2): 239-244

Molecular network of miR-106b-5p regulating osteoporotic fracture in the elderly

Wang Hailong1, Li Long1, Yilihamu Tuoheti1, Liu Xu2, Nacikedaoerji2, Chen Hongtao2, Cui Yong3

1Department of Orthopedics, the Seventh Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China; 2Department of Sports Injury, the Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830010, Xinjiang Uygur Autonomous Region, China; 3Department of Orthopedics, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China

Received:2020-09-28 Revised:2020-09-30 Accepted:2020-12-09 Online:2022-01-18 Published:2021-10-27
Contact: Cui Yong, Department of Orthopedics, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
About author:Wang Hailong, Department of Orthopedics, the Seventh Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
Supported by:
the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2017D01C276 (to CY)

Abstract: BACKGROUND: Osteoporotic fractures are age-related diseases, with increasing incidence and prevalence due to the aging population.
OBJECTIVE: To identify whether miRNA is associated with osteoporotic fracture.
METHODS: The differential expression of miRNA (DEM) related to osteoporotic fracture in GSE93883 was analyzed by limma package. The target genes of differentially expressed miRNA were predicted by RAID database, and the key miRNAs and target genes were identified by protein-protein interaction network. Enrichment analysis identified the biological functions and signaling pathways involved in target genes. Finally, qPCR and western blot were used to verify the key miRNAs and signal pathways in bone tissue of five elderly patients with osteoporotic fractures and five elderly patients with non-osteoporotic fractures.

RESULTS AND CONCLUSION: A total of 21 differentially expressed miRNAs related to osteoporotic fracture were obtained and 530 target genes were predicted. MiR-106b-5p was identified as a key regulatory gene by protein-protein interaction network. The target genes were mainly related to the activation of MAPK activity, PI3K-Akt signaling pathway and transforming growth factor β signaling pathway. In addition, qPCR results confirmed significant downregulation of miR-106b-5p in elderly osteoporotic fracture (P < 0.05). Western blot results showed that PI3K-Akt and transforming growth factor β signaling pathways were significantly activated in elderly osteoporotic fractures. Therefore, miRNA is involved in elderly osteoporotic fracture and is associated with PI3K-Akt and transforming growth factor βsignaling pathways. MiR-106b-5p may inhibit the occurrence of osteoporotic fractures and become a potential marker and therapeutic target.

Key words:osteoporotic fracture, miR-106b-5p, PI3K-Akt, transforming growth factor, differential expression

分享到：

Publishing Information

Publishing House of Chinese Journal of Tissue Engineering Research

The Official Publication of

Chinese Association of Rehabilitation Medicine

Contact Us

General editorial enquiries:

Email: bwb01@crter.org

Email: crter@crter.org

Commercial Sales contact (Reprints, advertising, etc.):

Email: bwb@crter.org