2022, Vol. 26 ›› Issue (21): 3363-3368

Effect of concentrated growth factor and bioceramic material iRoot BP on survival, proliferation and mineralization of human dental pulp cells in vitro

Yan Qifang, Xie Cuiliu, Yan Guowei   

Abstract: BACKGROUND: Concentrated growth factors are rich in a variety of high-concentration growth factors, which can induce the proliferation of dental pulp cells and promote the repair of local dentin.
OBJECTIVE: To compare the effects of concentrated growth factor and bioceramic material iRoot BP on the survival, proliferation and mineralization of human dental pulp cells in vitro, and then evaluate the feasibility of concentrated growth factor as a direct pulp capping material.
METHODS: The peripheral blood of healthy adults was collected and centrifuged to extract and concentrate growth factors. The modified tissue mass enzymatic digestion method was used to isolate and culture human dental pulp cells. The cells were treated with concentrated growth factor membrane extract and iRoot BP material extract. Cell proliferation was detected by the CCK8 assay. Human dental pulp cells were separately inoculated on the surface of the concentrated growth factor membrane and iRoot BP material to detect the alkaline phosphatase activity, cell cycle distribution, apoptosis and gene expression related to odontogenesis.
RESULTS AND CONCLUSION: (1) CCK8 assay showed that cell proliferation of the concentrated growth factor patch extract group was faster than that of the iRoot BP material extract group for 1, 3, and 7 days (P < 0.05). (2) The alkaline phosphatase activity of the cells of the concentrated growth factor group treated for 3 days was higher than that of the iRoot BP group (P < 0.05), and there was no significant difference in alkaline phosphatase activity between the two treatments for 1 and 7 days (P > 0.05). (3) The percentage of S-phase cells in the concentrated growth factor group treated for 1, 3, and 7 days was higher than that in the iRoot BP group (P < 0.05), and the apoptosis rate of cells on days 1, 3, and 7 was lower than that in iRoot BP group (P < 0.05). (4) The qRT-PCR test on the 7th day of treatment showed that the mRNA levels of Runx2, alkaline phosphatase, dentin sialophosphoprotein and osteocalcin in the concentrated growth factor group were higher than those in the iRoot BP group (P < 0.05). (5) The results show that concentrated growth factors can promote the survival, proliferation and mineralization of human dental pulp cells, and it is expected to be used as a direct pulp capping material.
Key words: platelets, concentrated growth factors, iRoot BP, odontogenic differentiation, direct pulp capping, dental pulp cells, autologous biological materials, platelet-rich plasma