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2022, Vol. 26 ›› Issue (24): 3892-3896

Expression of vasohibin-1 and other factors after the co-culture of two kinds of Echinococcus granulosus protoscolex and endothelial progenitor cells

Yu Xiaofan1, Jiang Huijiao2, Tan Xiaowu2, Wu Xiangwei2   

  1. 1School of Medicine, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China; 2Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China

  • Received:2021-04-07 Accepted:2021-05-21 Online:2022-08-28 Published:2022-01-24

  • Contact: Wu Xiangwei, MD, Professor, Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China

  • About author:Yu Xiaofan, Master, School of Medicine, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China

  • Supported by:

    the National Natural Science Foundation of China, No. 81760570 (to WXW)


Abstract: BACKGROUND: Alveolar echinococcosis and cystic echinococcosis grow in different ways in the body. Alveolar echinococcosis is infiltrating growth, which can invade tissues, blood vessels, and bile ducts. Cystic echinococcosis has fibrous outer cyst wall-wrapped lesions, which show compressive growth of tissues. The effect of the two types of hydatidosis on the invasion and formation of blood vessels is still unclear.  
OBJECTIVE: To explore the effects of two kinds of Echinococcus granulosus protoscolex on angiogenesis-related factors in endothelial progenitor cells.
METHODS:   After extraction, culture, and identification of endothelial progenitor cells from C57BL/6 mouse bone marrow, and extraction of two types of Echinococcus granulosus protoscolex, two kinds of Echinococcus granulosus protoscolex were co-cultured with endothelial progenitor cells for 24, 48, and 60 hours. ELISA was applied to detect the secretion of vascular endothelial growth factor A and stromal cell-derived factor-1α in the supernatant of cell co-culture. Western blot assay was utilized to detect the relative expression of vasohibin-1, vascular endothelial growth factor A, hypoxia-inducible factor 1α, and stromal cell-derived factor-1α protein in   endothelial progenitor cells of each group.  
RESULTS AND CONCLUSION: (1) The levels of vascular endothelial growth factor A and stromal cell-derived factor-1α were significantly higher in the alveolar echinococcosis group than those in the cystic echinococcosis group at 60 hours of coculture (P < 0.05). (2) Relative expression levels of vasohibin-1,   hypoxia-inducible factor 1α, and stromal cell-derived factor-1α were higher in the alveolar echinococcosis group than those in the cystic echinococcosis group at 48 and 60 hours of coculture (P < 0.05). (3) The relative expression levels of vascular endothelial growth factor A were not significantly different in the alveolar echinococcosis and cystic echinococcosis groups at 48 and 60 hours of coculture (P > 0.05). (4) It is concluded that alveolar echinococcosis promotes angiogenesis, and cystic echinococcosis has little effect on angiogenesis.
Key words: endothelial progenitor cells, alveolar echinococcosis, cystic echinococcosis, vasohibin-1, vascular endothelial growth factor, hypoxia-inducible factor 1α

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