2022, Vol. 26 ›› Issue (25): 3951-3955

LncRNA-HOTAIR can regulate differentiation of adipose derived mesenchymal stem cells into osteoblasts

Huang Tao1, Jia Zhiqiang2, Zhao Xiaoguang1, Wang Lei1, Fang Liping3, Zhai Wenjing4, Zhai Shafei5, Zhou Yongxin1

1First Affiliated Hospital of Xi’an Medical College, Xi’an 710077, Shaanxi Province, China; 2Second Affiliated Hospital of Henan University of Science and Technology, Luoyang 471000, Henan Province, China; 3Xi’an People’s Hospital, Xi’an 710068, Shaanxi Province, China; 4Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China; 5School of Stomatology, Xi’an Medical College, Xi’an 710021, Shaanxi Province, China

Received:2021-01-28 Accepted:2021-04-10 Online:2022-09-08 Published:2022-01-25
Contact: Zhou Yongxin, Master, Attending physician, First Affiliated Hospital of Xi’an Medical College, Xi’an 710077, Shaanxi Province, China
About author:Huang Tao, Master, Attending physician, First Affiliated Hospital of Xi’an Medical College, Xi’an 710077, Shaanxi Province, China
Supported by:
the National Natural Science Foundation of China, No. 81801237 (to HT)

Abstract: BACKGROUND: Evidence has shown that LncRNA-HOTAIR can regulate the expression of osteogenic related genes in bone marrow mesenchymal stem cells, but its effect on the osteogenic differentiation of adipose derived mesenchymal stem cells has not been reported.
OBJECTIVE: To investigate the effect of LncRNA-HOTAIR on the osteogenic differentiation of adipose derived mesenchymal stem cells.
METHODS: Adipose derived mesenchymal stem cells of Lewis rats were cultured in vitro. LncRNA-NC, lncRNA-HOTAIR mimics, and lncRNA-HOTAIR inhibitor were transfected into adipose derived mesenchymal stem cells and osteogenesis was induced. The alkaline phosphatase activity was detected on the 7th day of osteogenic induction. Alizarin red staining was performed on the 14th day of osteogenic induction. On the 6th day of osteogenic induction, the expression levels of osteogenic-related genes alkaline phosphatase, Runt-related transcription factor 2, osteocalcin, and osteopontin mRNA and protein were detected by RT-PCR and western blot assay.
RESULTS AND CONCLUSION: (1) After LncRNA-HOTAIR mimic transfection, alkaline phosphatase activity and calcification degree of adipose derived mesenchymal stem cells were significantly decreased, while LncRNA-HOTAIR inhibitor transfection significantly increased (P < 0.05). (2) After LncRNA-HOTAIR mimic transfection, the expression of alkaline phosphatase, Runt-related transcription factor 2, osteocalcin, and osteopontin in adipose derived mesenchymal stem cells decreased significantly (P < 0.05), but increased significantly after LncRNA-HOTAIR inhibitor transfection (P < 0.05). (3) These results suggest that LncRNA-HOTAIR can negatively regulate the osteogenic differentiation of adipose derived mesenchymal stem cells.
Key words: stem cells, adipose derived mesenchymal stem cells, LncRNA-HOTAIR, mimic, inhibitor, osteogenic differentiation

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