2022, Vol. 26 ›› Issue (25): 4010-4015

Mechanism of human umbilical cord mesenchymal stem cells to promote platelet plasma coagulation

Liu Chao1, 2, Zhang Lijun1, Du Xinjie1, Xu Qian3, Lü Hongjuan3, Fan Dongmei3, Tian Huanling2, Huang Jian2, Huang Yuxiang2   

Abstract: BACKGROUND: With the in-depth study of clinical application of mesenchymal stem cell, the clinical use of mesenchymal stem cell has gradually changed. To enhance the role of mesenchymal stem cell in local tissues, stem cell is generally used in combination with local injection of gel, and autologous platelet-rich plasma is an ideal gel replacement material.
OBJECTIVE: To explore the mechanism of human umbilical cord mesenchymal stem cells to promote platelet plasma coagulation.
METHODS: (1) Stem cell was mixed with anticoagulated plasma. Calcium chloride was used to stimulate coagulation. Clotting time was recorded. The effect of the number of human umbilical cord mesenchymal stem cells on the clotting time of plasma was analyzed. (2) CD142 expression in stem cell was analyzed by flow cytometry. CD142 antibody was used to pretreat human umbilical cord mesenchymal stem cells and to ensure if CD142 was one of the most important factors to improve plasma coagulation. (3) CD142 expressing model and regulation model were analyzed by checking on database. WGCNA and text mining were used to achieve the result why CD142 gene expression level was high in human umbilical cord mesenchymal stem cells.
RESULTS AND CONCLUSION: (1) Platelet plasma coagulation could be accelerated by CD142 expression of human umbilical cord mesenchymal stem cells. CD142 expression was enhanced as the increase in mesenchymal stem cell passage. (2) CD142 upregulation was a result of mesenchymal stem cell autocrine. (3) CD142 could affect the ability of cell chemotaxis and colonization in human umbilical cord mesenchymal stem cells.
Key words: stem cells, mesenchymal stem cells, umbilical cord, platelet plasma, platelet-rich plasma, CD142, coagulation, plasma coagulation