2022, Vol. 26 ›› Issue (27): 4325-4329

Effect of nanonized freshwater pearl powder on the expression of osteogenic related genes

Liao Jun, Xu Pu   

Abstract: BACKGROUND: The high content of calcium ions in pearls can promote calcium salt deposition, inhibit the bone resorption activity of osteoclasts, and promote bone regeneration. The water-soluble protein contained in pearls has an osteoinductive effect and can promote the differentiation of osteoblasts.
OBJECTIVE: To observe the effect of nanonized freshwater pearl powder on the expression of osteogenic related genes in osteoblasts.
METHODS: The mouse osteoblast MC3T3-E1 cells at passage 3 were taken and co-cultured with nano freshwater pearl powder (experimental group) and nano-hydroxyapatite (control group). The cells cultured separately were used as negative controls. At 7 days after culture, RT-PCR test was adopted to test the mRNA expression of Runx-related transcription factor II (Runx 2), osteopontin, and collagen type I.
RESULTS AND CONCLUSION: (1) The mRNA expression of Runx2 and osteopontin increased in the experimental group and control group compared with the negative control group (P < 0.05). The mRNA expression of Runx2 and osteopontin was higher in the experimental group than that in the control group (P < 0.05). (2) The mRNA expression of collagen type I was higher in the experimental group than that in the control group and negative control group (P < 0.05). The difference in mRNA expression of collagen type I was not significantly different between the control group and negative control group (P > 0.05). (3) These results suggest that nanonized freshwater pearl powder can significantly promote the expression of Runx2, osteopontin, and collagen type I mRNA better than nano-hydroxyapatite.
Key words: nanonized freshwater pearl powder, nanonized hydroxyapatite, osteoblast, osteogenic related genes, osteopontin, collagen type I