Journal Info

Journal Info

副标题

For Authors

For Authors

副标题

For Reviewers

For Reviewers

副标题

2022, Vol. 26 ›› Issue (29): 4611-4615

Recombinant human interferon alpha-2b inhibits the proliferation and migration of scar fibroblasts

Wang Rui1, Yu Fei1, Liu Taibin1, Hou Xiuli1, Liu Zhendong1, 2, Huo Jing1, Song Jianbo1   

  1. 1Dezhou People’s Hospital, Dezhou 253000, Shandong Province, China; 2Clinical Medical School of Yangzhou University/Northern Jiangsu People’s Hospital, Yangzhou 225001, Jiangsu Province, China

  • Received:2021-08-14 Accepted:2021-10-11 Online:2022-10-18 Published:2022-03-27

  • Contact: Huo Jing, Master, Associate chief physician, Dezhou People’s Hospital, Dezhou 253000, Shandong Province, China Song Jianbo, Master, Associate chief physician, Dezhou People’s Hospital, Dezhou 253000, Shandong Province, China

  • About author:Wang Rui, Master, Attending physician, Dezhou People’s Hospital, Dezhou 253000, Shandong Province, China

  • Supported by:

    the General Project of Shandong Provincial Medical and Health Technology Development Plan, No. 202004121375 (to WR)


Abstract: BACKGROUND: Recombinant human interferon α-2b is a cytokine with antiviral, antiproliferative, antitumor, and immunomodulatory activities, which has certain preventive and curative effects on scar tissue.  
OBJECTIVE: To explore the effects of recombinant human interferon α-2b on scar fibroblasts and to observe its effects on the proliferation and migration abilities of scar fibroblasts.  
METHODS: In vitro scar fibroblasts were cultured to logarithmic growth phase and treated with 0, 5 000, 10 000, 20 000 IU/mL recombinant human interferon α-2b for 48 hours. Cell proliferation was observed by EdU staining and cell migration was detected through cell scratch test and Transwell test. Expression levels of proliferating cell nuclear antigen and matrix metalloproteinase 2, proliferation- and migration-associated proteins of scar fibroblasts were detected by western blot assay.  
RESULTS AND CONCLUSION: The results of in vitro cell experiments showed that, compared with the control group, recombinant human interferon α-2b could significantly decrease the number of EdU-positive cells, the wound healing rate, the number of migrated cells, and the protein levels of proliferating cell nuclear antigen and matrix metalloproteinase 2 (P < 0.05). All these findings indicate that recombinant human interferon α-2b can inhibit the proliferation and migration of scar fibroblasts.
Key words: recombinant human interferon α-2b, hypertrophic scar, fibroblast, cell migration


分享到:

Publishing Information

Publishing House of Chinese Journal of Tissue Engineering Research


The Official Publication of

Chinese Association of Rehabilitation Medicine

Contact Us

General editorial enquiries:

Email: bwb01@crter.org

Copyright related contact:

Email: crter@crter.org

Commercial Sales contact (Reprints, advertising, etc.):

Email: bwb@crter.org