2022, Vol. 26 ›› Issue (30): 4762-4766

Expression of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence

Zhan Yuanbo1, Liu Xinpeng2, Xu Wenxia2, Miao Nan1, Mu Sen1, Zhang Ruimin1, Li Ying2   

Abstract: BACKGROUND: Bone marrow mesenchymal stem cells have aging phenomenon during in vitro culture, but the mechanism is still unclear. Histone deacetylases can deacetylate histones and make the chromatin condense, further shutting down genes.  
OBJECTIVE: To investigate the expression pattern of histone deacetylase 9 in bone marrow mesenchymal stem cells during senescence.
METHODS: The expression of histone deacetylases in bone marrow mesenchymal stem cells was analyzed by bioinformatics. Bone marrow mesenchymal stem cells were cultured by in vitro subculture. The senescence status of bone marrow mesenchymal stem cells was detected by β-galactosidase staining and qRT-PCR. The expression pattern of histone deacetylase 9 was detected by qRT-PCR, western blot assay, and immunofluorescence.  
RESULTS AND CONCLUSION: (1) Data mining and bioinformatics analysis showed that only histone deacetylase 9 expression was up-regulated among all histone deacetylase subtypes during bone marrow mesenchymal stem cell senescence. (2) Bone marrow mesenchymal stem cells presented obvious senescence at passage 10; for example, β-galactosidase-positive cells were the most obvious, and the expression of senescence-related genes was up-regulated. (3) The expression of histone deacetylase 9 was up-regulated and consistent with the data mining results. Histone deacetylase 9 was expressed in both the nucleus and cytoplasm of young bone marrow mesenchymal stem cells, but the expression in the nucleus was lower than that in the nucleus of senescence bone marrow mesenchymal stem cells. The expression of histone deacetylase 9 was almost not expressed in the cytoplasm of senescence bone marrow mesenchymal stem cells, but increased in the nucleus. (4) The results have shown that histone deacetylase 9 plays an important role in the senescence process of bone marrow mesenchymal stem cells, but the aging phenotype and molecular mechanism of histone deacetylase 9 need to be further studied.
Key words: stem cells, bone marrow mesenchymal stem cells, cellular senescence, natural aging, histone deacetylase 9