2022, Vol. 26 ›› Issue (32): 5167-5172

Microtubule-associated protein 1 light chain 3 is involved in the occurrence and development of steroid-induced avascular necrosis of the femoral head in a rabbit model

Ma Hailong1, Zhao Zhenqun2, Liu Wanlin2, Sun Jun1

1Department of Pediatric Orthopedics, Anhui Provincial Children’s Hospital, Children’s Hospital of Anhui Medical University, the Fifth Clinical Medical College of Anhui Medical University, Hefei 230051, Anhui Province, China; 2Department of Pediatric Orthopedics, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China

Received:2021-12-01 Accepted:2022-01-18 Online:2022-11-18 Published:2022-05-14
Contact: Sun Jun, Master, Professor, Doctoral supervisor, Department of Pediatric Orthopedics, Anhui Provincial Children’s Hospital, Children’s Hospital of Anhui Medical University, the Fifth Clinical Medical College of Anhui Medical University, Hefei 230051, Anhui Province, China Liu Wanlin, Master, Professor, Doctoral supervisor, Department of Pediatric Orthopedics, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
About author:Ma Hailong, MD candidate, Attending physician, Department of Pediatric Orthopedics, Anhui Provincial Children’s Hospital, Children’s Hospital of Anhui Medical University, the Fifth Clinical Medical College of Anhui Medical University, Hefei 230051, Anhui Province, China Zhao Zhenqun, MD, Chief physician, Master’s supervisor, Department of Pediatric Orthopedics, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
Supported by:
the National Natural Science Foundation of China, Nos. 81360273 (to LWL), 81460331 (to ZZQ and LWL [project participants]), 81560349 (to LWL) and 81760391 (to ZZQ)

Abstract: BACKGROUND: Steroid-induced avascular necrosis of the femoral head is still a difficult problem, and it is of great significance to study its pathogenesis.
OBJECTIVE: To discuss the role of autophagy gene microtubule-associated protein 1 light chain 3 (MAP1LC3) in steroid-induced avascular necrosis of the femoral head and the effect of 3-methyladenine on MAP1LC3.
METHODS: A total of 36 healthy New Zealand white rabbits, aged 5 months, were randomly divided into 3 groups, with 12 rabbits in each group. Control group was given intramuscular injection of normal saline, 2 mL per rabbit, once a week, four times in total. Model group was given intramuscular injection of 4 mg/kg methylprednisolone, once a week, four times in total. 3-Methyladenine group was given intramuscular injection of 4 mg/kg methylprednisolone followed by intramuscular injection of 3-methyladenine, 2 μL per rabbit, once a week, four times in total. Three animals from each group were sacrificed at 1, 2, 3, and 4 weeks for sampling. The femoral heads of the bilateral hindlimbs were taken. Empty bone lacunae were counted under optical microscope. mRNA and protein expression levels of MAP1LC3 were detected by RT-PCR and western blot assay, respectively.
RESULTS AND CONCLUSION: Compared with the control group, the rate of empty lacunae increased significantly in the model and 3-methyladenine groups (P < 0.05), but the rate of empty lacunae was significantly lower in the 3-methyladenine group than the model group (P < 0.05). The expression of MAP1LC3 mRNA in the model group was higher than that in the 3-methyladenine group (P < 0.05), indicating the activation of autophagy is inhibited by 3-methyladenine. These findings indicate that MAP1LC3 is expressed in the animal model, confirming that autophagy plays a certain role in the pathogenesis of steroid-induced avascular necrosis of the femoral head; 3-methyladenine inhibits the expression of MAP1LC3 and reduces the occurrence and development of steroid-induced avascular necrosis of the femoral head.
Key words: steroid-induced avascular necrosis of the femoral head, autophagy, MAP1LC3, 3-methyladenine, animal model

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