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2022, Vol. 26 ›› Issue (4): 516-520Biodentine enhances the proliferation and differentiation of osteoblasts through upregulating bone morphogenetic protein-2Yang Sidi1, 2, Wang Qian1, Xu Nuo3, Wang Ronghan1, Jin Chuanqi1, Lu Ying1, Dong Ming1
Abstract: BACKGROUND: Biodentine is a new kind of biocompatible calcium silicate dental restoration material. It can not only increase the secretion of growth factors in dental pulp cells, but also induce the mineralization of dentin. However, the research of Biodentine in bone repair is few. OBJECTIVE: To investigate the effects of Biodentine on the proliferation and differentiation of bone morphogenetic protein-2 interfered mouse osteoblast precursor cell lines by constructing mouse osteoblast precursor cell lines in which bone morphogenetic protein-2 was interfered. METHODS: The 0.1, 1, 10, and 100 g/L Biodentine extract was applied to mouse osteoblast precursor cells for 24 hours. The optimal concentration of 10 g/L was selected by CCK-8 and alkaline phosphatase for the following experiments. Biodentine extract was used to treat mouse osteoblast precursor cells for 1, 3, 5 and 7 days, and the non-intervention group was used as the control group. The cell proliferation and osteogenic differentiation were observed by CCK-8 assay and alkaline phosphatase activity test. Mouse osteoblast precursor cell lines were constructed by interfering with bone morphogenetic protein-2. The proliferation and osteogenic differentiation of cells were observed by CCK-8 assay and alkaline phosphatase activity test. The mouse osteoblast precursor cell lines interfering with bone morphogenetic protein-2 were constructed. The Biodentine group was added with 10 g/L Biodentine extract, and the non-intervention group was set as the control. After 24 and 48 hours of treatment, the proliferation and osteogenic differentiation of the cells were observed by CCK-8 assay and alkaline phosphatase activity test. After 24 hours of treatment, the expression levels of alkaline phosphatase mRNA and protein were detected by real-time PCR and western blot assay. RESULTS AND CONCLUSION: (1) 10 g/L Biodentine extract could promote the proliferation and osteogenic differentiation of mouse osteoblast progenitor cells at 1, 3, 5 and 7 days. (2) Interfering with bone morphogenetic protein-2 could inhibit the proliferation and osteogenic differentiation of mouse osteoblast progenitor cells. (3) The cell proliferation and osteogenic differentiation abilities of Biodentine group were higher than those of control group at 24 and 48 hours (P < 0.05), and the expression levels of alkaline phosphatase mRNA and protein of Biodentine group were higher than those of the control group (P < 0.05). (4) The results showed that 10 g/L Biodentine could enhance the proliferation and osteogenic differentiation of osteoblasts through bone morphogenetic protein-2. Key words:Biothentine, calcium silicate material, osteoblast precursor cell, MC3T3-E1 cell, bone morphogenetic protein-2, cell proliferation, cell differentiation |