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2022, Vol. 26 ›› Issue (5): 688-693

Ermiao san aqueous extract regulates proliferation, migration, and inflammatory factor expression of fibroblast-like synovial cells in collagen-induced arthritis rats

Liu Jin1, Li Zhen1, 2, Hao Huiqin1, 2, Wang Ze1, Zhao Caihong1, Lu Wenjing1     

  1. 1Basic Laboratory of Integrated Traditional Chinese and Western Medicine, 2School of Basic Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China

  • Received:2020-11-23 Revised:2020-11-28 Accepted:2020-12-24 Online:2022-02-18 Published:2021-10-28

  • Contact: Hao Huiqin, MD, Professor, Chief physician, Basic Laboratory of Integrated Traditional Chinese and Western Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China; School of Basic Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China

  • About author:Liu Jin, Master candidate, Basic Laboratory of Integrated Traditional Chinese and Western Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China

  • Supported by:

    the National Natural Science Foundation of China (Youth Science Fund Project), No. 81904034 (to LZ); Shanxi Province Graduate Student Innovation Project, No. 2019SY519 (to LJ); Shanxi University of Chinese Medicine Graduate Student Innovation Project, No. 2020CX028 (to LJ); Fundamental Research Project of Shanxi University of Chinese Medicine, No. 2020PY-JC(Y)-07 and 2020PY-JC(Y)-08 (to LZ and HHQ); Shanxi University of Chinese Medicine Science and Technology Innovation Team Key Project, No. 2018TD-011 (to HHQ)



Abstract: BACKGROUND: The activation of nuclear factor -κB signaling pathway is closely related to the proliferation and inflammation of fibroblast-like synovial cells in rheumatoid arthritis. However, the mechanism by which Ermiao san aqueous extract interferes with fibroblast-like synoviocytes of collagen-induced arthritis rats (CIA-FLS) via the NF-κB signaling pathway has not been reported.
OBJECTIVE: To study the effects of different concentrations of Ermiao san aqueous extracts on the proliferation, migration and inflammatory factors expression of CIA-FLS, and to explore its mechanism based on the nuclear factor-κB signaling pathway.
METHODS: CIA-FLS cells were resuscitated and coped with different concentrations of Ermiao san aqueous extracts (200, 400, 800, 1600, 3 200 mg/L) after subculture. The proliferation and migration ability of CIA-FLS were detected by MTT method and Transwell chamber, and real-time fluorescent quantitative PCR (qRT-PCR) and western blot assay were used to detect the NF-κB signaling pathway related molecules interleukin-6, tumor necrosis factor-α, nuclear factor-κBp65 mRNA and protein expression levels in CIA-FLS.

RESULTS AND CONCLUSION: Different concentrations of Ermiao san aqueous extracts acted on CIA-FLS for 48 hours. Compared with the blank control group, Ermiao san aqueous extracts inhibited the proliferation of CIA-FLS in a dose-dependent manner (P < 0.05). 1 600 mg/L Ermiao san aqueous extract significantly inhibited the proliferation and migration of CIA-FLS (P < 0.01), and at the same time, it significantly inhibited the expression of interleukin-6, tumor necrosis factor-α, nuclear factor-κBp65 mRNA and protein in CIA-FLS (P < 0.01). Therefore, the optimal concentration of Ermiao san aqueous extract to inhibit the proliferation, migration and inflammatory factor expression of CIA-FLS is 1 600 mg/L. Ermiao san aqueous extract may inhibit the activation of nuclear factor-κB signaling pathway and the production of inflammatory factors, thereby reducing the proliferation and inflammation of CIA-FLS. It provides certain research experimental basis for further research on the mechanism of Ermiao san treatment of rheumatoid arthritis in the later period.

Key words:Ermiao San aqueous extract, fibroblast-like synoviocytes, rheumatoid arthritis, inflammatory factor, nuclear factor-κB, signaling pathway


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