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2022, Vol. 26 ›› Issue (5): 688-693Ermiao san aqueous extract regulates proliferation, migration, and inflammatory factor expression of fibroblast-like synovial cells in collagen-induced arthritis ratsLiu Jin1, Li Zhen1, 2, Hao Huiqin1, 2, Wang Ze1, Zhao Caihong1, Lu Wenjing1
Abstract: BACKGROUND: The activation of nuclear factor -κB signaling pathway is closely related to the proliferation and inflammation of fibroblast-like synovial cells in rheumatoid arthritis. However, the mechanism by which Ermiao san aqueous extract interferes with fibroblast-like synoviocytes of collagen-induced arthritis rats (CIA-FLS) via the NF-κB signaling pathway has not been reported. OBJECTIVE: To study the effects of different concentrations of Ermiao san aqueous extracts on the proliferation, migration and inflammatory factors expression of CIA-FLS, and to explore its mechanism based on the nuclear factor-κB signaling pathway. METHODS: CIA-FLS cells were resuscitated and coped with different concentrations of Ermiao san aqueous extracts (200, 400, 800, 1600, 3 200 mg/L) after subculture. The proliferation and migration ability of CIA-FLS were detected by MTT method and Transwell chamber, and real-time fluorescent quantitative PCR (qRT-PCR) and western blot assay were used to detect the NF-κB signaling pathway related molecules interleukin-6, tumor necrosis factor-α, nuclear factor-κBp65 mRNA and protein expression levels in CIA-FLS. RESULTS AND CONCLUSION: Different concentrations of Ermiao san aqueous extracts acted on CIA-FLS for 48 hours. Compared with the blank control group, Ermiao san aqueous extracts inhibited the proliferation of CIA-FLS in a dose-dependent manner (P < 0.05). 1 600 mg/L Ermiao san aqueous extract significantly inhibited the proliferation and migration of CIA-FLS (P < 0.01), and at the same time, it significantly inhibited the expression of interleukin-6, tumor necrosis factor-α, nuclear factor-κBp65 mRNA and protein in CIA-FLS (P < 0.01). Therefore, the optimal concentration of Ermiao san aqueous extract to inhibit the proliferation, migration and inflammatory factor expression of CIA-FLS is 1 600 mg/L. Ermiao san aqueous extract may inhibit the activation of nuclear factor-κB signaling pathway and the production of inflammatory factors, thereby reducing the proliferation and inflammation of CIA-FLS. It provides certain research experimental basis for further research on the mechanism of Ermiao san treatment of rheumatoid arthritis in the later period. Key words:Ermiao San aqueous extract, fibroblast-like synoviocytes, rheumatoid arthritis, inflammatory factor, nuclear factor-κB, signaling pathway |