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2022, Vol. 26 ›› Issue (7): 1032-1037Plant-derived basic fibroblast growth factor maintains the growth and differentiation of human embryonic stem cellsLiu Feng1, 2, Peng Yuhuan3, Luo Liangping1, Wu Benqing4
Abstract: BACKGROUND: Basic fibroblast growth factor is an important growth factor to maintain the long-term culture of human embryonic stem cells. Providing reliable, non-animal-derived and low-cost basic fibroblast growth factor products is essential for maintaining the growth of human embryonic stem cells and large-scale production. OBJECTIVE: To eliminate the potential interference of animal or other bacteria, this study mainly evaluated the effects of plant-derived basic fibroblast growth factor on the growth and differentiation of human embryonic stem cells. METHODS: Human embryonic stem cells line (H9) was used as research materials. Immunofluorescence staining, flow cytometry detection, alkaline phosphatase staining, and RT-PCR were utilized to systematically compare the ability of plant-derived basic fibroblast growth factor to maintain the characteristics of stem cells at different concentrations and its potential to differentiate into ectoderm, mesoderm, endoderm and pancreatic cells. RESULTS AND CONCLUSION: Plant-derived basic fibroblast growth factor (4 μg/L and 0.4 μg/L) and basic fibroblast growth factor (4 μg/L) used in conventional culture system could maintain the growth and differentiation of human embryonic stem cells, indicating that plant-derived basic fibroblast growth factor can be used as an effective alternative product to support the growth and differentiation of human embryonic stem cells. Key words:stem cells, human embryonic stem cells, basic fibroblast growth factor, plant-derived, differentiation, pancreatic cells |