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2022, Vol. 26 ›› Issue (7): 1038-1044Nocardia rubra cell wall skeleton for extemal use improves the viability of adipogenic mesenchymal stem cells and promotes diabetes wound repairWen Dandan1, Li Qiang2, Shen Caiqi2, Ji Zhe1, Jin Peisheng2
Abstract: BACKGROUND: According to the current clinical application of Nocardia rubra cell wall skeleton to the treatment of cervical erosion and its immune effect, considering the possibility of its treatment of chronic wounds. OBJECTIVE: To study the effect of Nocardia rubra cell wall skeleton for extemal use on the proliferation and apoptosis of adipogenic mesenchymal stem cells in vitro, and the impact on the survival rate and skin healing of adipogenic mesenchymal stem cells transplantation in vivo. METHODS: Adipogenic mesenchymal stem cells were treated with 10 mg/L Nocardia rubra cell wall skeleton for extemal use. CCK-8 and EDU kits were used to detect the viability and proliferation of adipogenic mesenchymal stem cells. Adipogenic mesenchymal stem cells were treated with 50% sucrose to induce apoptosis, and then with Nocardia rubra cell wall skeleton for extemal use. Afterwards, flow cytometry and TUNEL assay were used to detect the apoptosis. Nocardia rubra cell wall skeleton for extemal use (10 mg/L, 5 mL) was added to passage 3 adipogenic mesenchymal stem cells for 48 hours of culture. The pretreated cells labeled with the fluorescent dye CM-Dil were injected intracutaneously into the wound skin of diabetic mice. The cell survival rate was detected by the LB983 in vivo imaging system. Hematoxylin-eosin staining, Masson staining, and immunofluorescence were used to verify the healing of chronic wounds on day 14. RESULTS AND CONCLUSION: Nocardia rubra cell wall skeleton for extemal use could promote the viability and proliferation of adipogenic mesenchymal stem cells, and inhibit their apoptosis. Nocardia rubra cell wall skeleton for extemal use increased the survival rate of adipogenic mesenchymal stem cells under in vivo conditions. The wound healing rate is faster in diabetic mice. Key words:Nocardia rubra cell wall skeleton, adipogenic mesenchymal stem cells, cell viability, apoptosis, chronic wound, diabetic wounds |