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2022, Vol. 26 ›› Issue (7): 1045-1050Interleukin-8 receptor enhances the migration and adhesion of umbilical cord mesenchymal stem cells to injured endotheliumZhu Bingbing1, 2, Deng Jianghua2, Chen Jingjing2, Mu Xiaoling2
Abstract: BACKGROUND: Endothelial dysfunction is an early predictor of atherosclerosis and cardiovascular disease. Umbilical cord mesenchymal stem cell transplantation provides the possibility for the treatment of vascular injury. OBJECTIVE: To explore the effect of overexpression of interleukin-8 receptor A/B on migration of human umbilical cord mesenchymal stem cells. METHODS: Primary cultured human umbilical cord mesenchymal stem cells and human umbilical vein endothelial cells were transfected with adenovirus vector containing interleukin-8 receptor A/B. Confocal immunofluorescence microscopy and western blot assay were used to observe the expression of interleukin-8 receptor A/B in cells. CCK-8 assay, competition test and adhesion test were used to detect the effects of interleukin-8 receptor A/B overexpression on cell proliferation, competitiveness and migration. RESULTS AND CONCLUSION: The fluorescent adenovirus vector overexpressing interleukin-8 receptor A/B has no significant effect on cell proliferation. However, the competition and migration abilities of human umbilical cord mesenchymal stem cells overexpressing interleukin-8 receptor A/B were significantly higher than those of human umbilical vein endothelial cells overexpressing interleukin-8 receptor A/B (P < 0.05). It is concluded that interleukin-8 receptor enhances the migration ability of human umbilical cord mesenchymal stem cells and promotes adhesion to injured endothelium. Key words: stem cells, human umbilical cord mesenchymal stem cells, human umbilical vein endothelial cells, interleukin-8 receptor, adenovirus, proliferation, competition, adhesion |