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2022, Vol. 26 ›› Issue (7): 1068-1073Effects of serum containing Wuzang Wenyang Huayu Decoction on phosphorylated-tau protein expression in Alzheimer’s disease cell modelWen Xiaoyu, Sun Yuhao, Xia Meng
Abstract: BACKGROUND: Various previous studies have shown that Wuzang Wenyang Huayu Decoction has a good therapeutic effect on Alzheimer’s disease, but its pharmacological mechanism has not been fully elucidated. OBJECTIVE: To investigate the effect of Wuzang Wenyang Huayu Decoction drug-containing serum on the expression of peroxisome proliferator-activated receptor γ and phosphorylated-tau protein in Alzheimer’s disease cell model. METHODS: Beta amyloid protein was used to induce primary hippocampal neurons to establish the currently recognized Alzheimer’s disease cell model. Wuzang Wenyang Huayu Decoction drug-containing serum and donepezil hydrochloride drug-containing serum were given for intervention for 72 hours. The dendritic length and branch number of primary neurons cells were detected by immunofluorescence assay. The expression levels of peroxisome proliferator-activated receptor γ and phosphorylated-tau protein were detected by western blot assay. RESULTS AND CONCLUSION: Compared with the control group, the length and branch number of dendritic cells in the model group were significantly decreased (P < 0.05), peroxisome proliferator-activated receptor γ expression decreased, and phosphorylated-tau protein increased (P < 0. 05 or P < 0. 01). Compared with the model group, the dendritic length and branch number of neurons in Wuzang Wenyang Huayu group and donepezil hydrochloride group increased, the expression of PPARγ increased, and the expression of phosphorylated-tau protein significantly decreased (P < 0. 05 or P < 0. 01). Results confirmed that Wuzang Wenyang Huayu Decoction has neuroprotective effect on Alzheimer’s disease cells induced by beta amyloid protein, and its mechanism may be related to upregulation of peroxisome proliferator-activated receptor γ protein and inhibition of phosphorylated-tau protein. Key words:neuron, Alzheimer’s disease, Wuzang Wenyang Huayu Decoction, beta amyloid protein, peroxisome proliferator-activated receptor γ, phosphorylated-tau protein |