2022, Vol. 26 ›› Issue (7): 999-1004

Osteogenic ability of adipose stem cells in diabetic osteoporosis mice

Gao Yujin1, Peng Shuanglin1, Ma Zhichao1, Lu Shi1, Cao Huayue1, Wang Lang1, Xiao Jingang1, 2, 3

1School of Stomatology of Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; 3Orofacial Reconstruction and Regeneration Laboratory of Southwest Medical University, Luzhou 646000, Sichuan Province, China

Received:2021-03-18 Revised:2021-03-20 Accepted:2021-04-23 Online:2022-03-08 Published:2021-10-29
Contact: Xiao Jingang, MD, Professor, School of Stomatology of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Orofacial Reconstruction and Regeneration Laboratory of Southwest Medical University, Luzhou 646000, Sichuan Province, China
About author:Gao Yujin, Master candidate, School of Stomatology of Southwest Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
the National Natural Science Foundation of China, No. 81870746 (to XJG); the Science and Technology Strategic Cooperation Project of Luzhou Municipal People’s Government-Southwest Medical University, No. 2020LZXNYDZ09 (to XJG)

Abstract: BACKGROUND: Long noncoding RNA (LncRNA)-DANCR can regulate multiple biological processes, including bone metabolism. Our gene microarray results showed that LncRNA-DANCR was significantly overexpressed in diabetic osteoporosis mice.
OBJECTIVE: To investigate the effect of LncRNA-DANCR on the osteogenic ability of adipose stem cells in diabetic osteoporosis through Wnt/β-catenin signaling pathway.
METHODS: Diabetic osteoporosis adipose-derived stem cells and control adipose-derived stem cells were isolated and cultured. After 3 days of osteogenic induction, real-time fluorescence quantitative PCR and western blot assay were used to examine the expression of LncRNA-DANCR, Wnt signaling molecules β-catenin and osteogenic transcription factors (RUNX2 and OPN). Diabetic osteoporosis adipose-derived stem cells transfected with lncRNA-DANCR-specific siRNA were designed. After 3 days of osteogenic induction, real-time fluorescence quantitative PCR, western blot assay and alkaline phosphatase staining were used to examine the expression of Wnt signaling molecules β-catenin, osteogenic transcription factors RUNX2 and OPN and osteogenic ability changes.

RESULTS AND CONCLUSION: (1) After 3 days of osteogenic induction, the expression level of LncRNA-DANCR in diabetic osteoporosis adipose-derived stem cells group was significantly higher than that in control group. The expression levels of Wnt signaling molecule β-catenin and osteogenic transcription factors RUNX2 and OPN were significantly lower than those in control adipose-derived stem cells. (2) After 3 days of osteogenic induction, β-catenin and RUNX2 and OPN were highly expressed in the siRNA silent group, and the ability of bone formation was enhanced. (3) The results confirmed that LncRNA-DANCR decreased the osteogenic capacity of diabetic osteoporosis adipose-derived stem cells by inhibiting the Wnt/β-catenin signaling pathway. Silencing of LncRNA-DANCR can restore the reduced osteogenic capacity.

Key words:long noncoding RNA, Wnt signaling pathway, diabetes, osteoporosis, osteogenic differentiation, bone repair

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