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2022, Vol. 26 ›› Issue (8): 1180-1186Miao medicine fumigation can delay extracellular matrix destruction in a rabbit model of knee osteoarthritisWang Baojuan1, Zheng Shuguang1, Zhang Qi2, Li Tianyang2
Abstract: BACKGROUND: Previous studies have shown that Miao medicine fumigation therapy has a definite effect on the treatment of osteoarthritis; however, its mechanism is unclear. Because excessive angiogenesis in synovial tissue and subchondral bone tissue is an important cause of the occurrence and development of osteoarthritis, vascular endothelial growth factor is an important pro-angiogenesis factor in the body, and the stromal cell-derived factor 1/CXCR4 signaling pathway is closely related to the overexpression of vascular endothelial growth factor. OBJECTIVE: To explore the role of stromal cell-derived factor 1/CXCR4 signaling pathway in the osteoarthritic occurrence and development and the therapeutic mechanism of Miao medicine fumigation on knee osteoarthritis. METHODS: Fifty healthy New Zealand white rabbit were randomized into normal, model, positive control (voltaren ointment for external use), early intervention, and Miao medicine fumigation groups. The knee osteoarthritis model was established in the latter four groups by injecting sodium iodoacetate into the knee joint cavity of rabbits. Hematoxylin-eosin staining was used to observe the pathological changes of synovium and cartilage. Immunohistochemical method was used to detect the levels of interleukin-1β and tumor necrosis factor-α in synovial tissue. ELISA method was used to detect the level of stromal cell-derived factor 1 in synovial fluid and the level of vascular endothelial growth factor in serum. Real-time fluorescence quantitative PCR was used to detect vascular endothelial growth factor mRNA expression in articular cartilage tissue. Western blot assay was used to detect the expression of type II collagen and aggrecan protein in cartilage tissue. RESULTS AND CONCLUSION: Compared with the model group, Miao medicine fumigation treatment significantly inhibited the inflammation in synovium and cartilage tissue and reduced its pathological changes. Compared with the model group, the integral absorbance values of interleukin-1β and tumor necrosis factor-α in synovium and the levels of vascular endothelial growth factor in serum and stromal cell-derived factor in joint fluid were significantly reduced in the Miao medicine fumigation group (P < 0.05). Compared with the model group, the serum vascular endothelial growth factor mRNA was reduced in the Miao medicine fumigation group, while type II collagen and aggrecan expressions were increased (P < 0.05). The above experimental results indicated no significant differences among Miao medicine fumigation group, positive control group and early intervention group (P > 0.05). To conclude, the Miao medicine fumigation therapy maybe by reducing the binding of stromal cell-derived factor 1 to its receptor CXCR4 inhibits the expression of interleukin-1β, tumor necrosis factor-α, and vascular endothelial growth factor, delays the destruction of extracellular matrix, and plays a therapeutic role. Key words:osteoarthritis, Miao medicine fumigation therapy, vascular endothelial growth factor, stromal cell-derived factor 1/CXCR4 signaling pathway, stromal cell-derived factor 1, rabbit, animal model |