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2022, Vol. 26 ›› Issue (8): 1228-1233Protective effect and mechanism of dendrobium on fibroblasts damaged by ultraviolet BLi Qin1, 2, Mao Shuangfa1, Li Min2, Cheng Jiyan2
Abstract: BACKGROUND: Ultraviolet B increases reactive oxygen species, which is the most important physical factor of light damage and photoaging. Dendrobium polysaccharides contained in dendrobium can improve the activity of antioxidant enzymes, scavenge free radicals, and inhibit lipid peroxidation, which have certain effects on stabilizing biofilms and delaying aging. OBJECTIVE: To investigate the preventive effect and mechanism of dendrobium on the photoaging fibroblasts of human induced by ultraviolet B in vitro. METHODS: Human fibroblasts cultured in vitro were randomized into four groups: a control group with normal culture, a model group irradiated by one-time ultraviolet B at a dose of 20 mJ/cm2, a dendrobium group interfered with 30 mg/L dendrobium polysaccharides, and an experimental group interfered with 30 mg/L dendrobium polysaccharides following 20 mJ/cm2 ultraviolet B irradiation. Cells in each group were then cultured for another 48 hours. Expression of Nrf2 and superoxide dismutase at gene and protein levels was detected using qRT-PCR, western blot and immunohistochemistry. Cellular reactive oxygen levels were detected using fluorescence detection, and malondialdehyde content was detected using colorimetric determination. RESULTS AND CONCLUSION: The mRNA and protein levels of Nrf2 and superoxide dismutase were significantly up-regulated in the model, dendrobium and experimental groups compared with the control group (P < 0.05). The mRNA and protein levels of Nrf2 and superoxide dismutase were significantly higher in the experimental group than the model and dendrobium groups (P < 0.05). Compared with the control group, the reactive oxygen level was significantly increased in the model group (P < 0.05), and decreased in the dendrobium group (P < 0.05). The reactive oxygen level in the experimental group was significantly lower than that in the model group (P < 0.05). Compared with the control group, the malondialdehyde content was significantly increased in the model and experimental groups (P < 0.05), and significantly decreased in the dendrobium group (P < 0.05). The malondialdehyde content in the experimental group was significantly lower than that in the model group (P < 0.05). To conclude, dendrobium has certain protective effects on the photoaging of human fibroblasts induced by ultraviolet B. The main mechanism of dendrobium may be to regulate the production of superoxide dismutase, and eliminate reactive oxygen, reduce cell damage caused by ultraviolet B through Keap1-Nrf2-ARE signaling pathway. Key words:photoagin, fibroblasts, dendrobium, dendrobium polysaccharides, superoxide dismutase, reactive oxygen, Nrf2, malondialdehyde |