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2022, Vol. 26 ›› Issue (8): 1242-1246High-dose vitamin C promotes the survival of autologous fat transplantation in ratsXiang Xinjian, Liu Fang, Wu Liangliang, Jia Daping, Tao Yue, Zhao Zhengnan, Zhao Yu
Abstract: BACKGROUND: Fat transplantation is often used for soft tissue filling, reconstruction, and beauty treatments. However, the survival rate of fat transplants varies greatly. How to improve the survival rate of fat transplants has become a research focus. OBJECTIVE: To explore the effect of different doses of vitamin C on the survival of fat transplants after autologous fat transplantation. METHODS: Twenty female Sprague-Dawley rats were randomized into a low-, high-, super-high-dose vitamin C groups and control group (n=5 per group). The abdominal mesenteric fat of rats in each group was taken out, and 0.8 g of fat particles was transplanted into a 1 cm diameter subcutaneous sac on the back of each rat after proper treatment. The experimental groups were intraperitoneally injected with 1 mL of vitamin C solution of 50, 150, and 1 000 mg/d respectively for 7 consecutive days after operation. The control group was intraperitoneally injected with the same amount of normal saline. After 8 weeks, the rats were euthanized. Then the grafts were taken out and evaluated as follows after removal of the adherent fibrous connective tissue: (1) gross observation; (2) retention weight; (3) hematoxylin-eosin staining; (4) immunohistochemical staining of CD34. RESULTS AND CONCLUSION: The texture of fat in the three vitamin C groups was generally softer than that of the control group, and there were more small blood vessels on the surface of the fat graft. The graft retention weight of the vitamin C groups was significantly higher than that of the control group, and the retention weight of the high-dose group was highest (P < 0.001). The formation of vesicles in the three vitamin C groups was reduced. High- and super-high-dose vitamin C was more effective in reducing inflammatory infiltration and fibrosis (P < 0.05). The capillary densities in the high- and super-high-dose vitamin C groups were higher than that in the control group (P < 0.001). To conclude, high-dose vitamin C can increase the capillary density and retention quality of autologous fat, reduce the degree of inflammatory infiltration and fibrosis, and reduce the formation of vesicles in rats. Key words:vitamin C, autologous fat transplantation, antioxidant, absorption of autograft, inflammation, fibrosis, optimal dosage, rat |