2023, Vol. 27 ›› Issue (10): 1528-1533

Lentiviral down-regulation of casein kinase 2-interacting protein-1 expression in bone marrow mesenchymal stem cells to repair alveolar bone defects in osteoporosis rats

Xie Mengsheng1, 2, Long Yanming1, 2, Li Xiaojie1, 2   

Abstract: BACKGROUND: Osteoporosis is a frequently encountered and urgent problem in the clinical repair and treatment of alveolar bone and often affects the treatment effect. Casein kinase 2-interacting protein-1 (CKIP-1) has recently been found to have a negative regulation of osteogenesis, so it is promising as a new target for the treatment of osteoporosis.
OBJECTIVE: To explore the repair effect of bone marrow mesenchymal stem cells modified with CKIP-1 on alveolar bone defects in osteoporosis rats.
METHODS:   (1) Bone marrow mesenchymal stem cells were isolated and cultured from 1-week-old SD rats. Lentiviral transfection reduced the expression of CKIP-1 in bone marrow mesenchymal stem cells. (2) The model of osteoporosis rats was established by intragastric administration of retinoic acid (70 mg/kg per day) for 21 days. The complex of bone marrow mesenchymal stem cells modified with CKIP-1 and gelatin sponge, the complex of bone marrow mesenchymal stem cells transfected with empty vector and gelatin sponge, and the gelatin sponge were implanted into the alveolar bone defect of the osteoporosis rat model, separately. Gelatin sponge was implanted into bilateral alveolar bone defects of normal rat model of the normal group. After 30 days of normal feeding, the maxillary alveolar bone was taken for sectioning and staining, and the healing of bone defect was calculated by the Lane-Sandhu histological score and the percentage of new bone. The relative mRNA expression and positive expression of CKIP-1, alkaline phosphatase and osteocalcin in the alveolar bone of each group were detected by qRT-PCR and immunohistochemical staining.
RESULTS AND CONCLUSION: (1) Compared with empty vector transfection, the relative expression of CKIP-1 mRNA in bone marrow mesenchymal stem cells modified with CKIP-1 decreased (P < 0.05). (2) Compared with the bone marrow mesenchymal stem cells transfected with the empty vector and gelatin sponge complex group and the gelatin sponge group, the Lane-Sandhu histological score and the percentage of new bone significantly increased in the bone marrow mesenchymal stem cells modified with CKIP-1 and gelatin sponge complex group (P < 0.05), which were similar to the normal group; the expression levels of alkaline phosphatase and osteocalcin were significantly increased (P < 0.05). (3) The results indicated that local transplantation of CKIP-1 transfected modified bone marrow mesenchymal stem cells had better repair effect on alveolar bone defects in osteoporosis rats.
Key words: osteoporosis, CKIP-1, bone marrow mesenchymal stem cell, tissue engineering, lentiviral transfection, bone defect, rat