Journal Info

Journal Info

副标题

For Authors

For Authors

副标题

For Reviewers

For Reviewers

副标题

2023, Vol. 27 ›› Issue (10): 1547-1552

Effects of chiral polyethyleneimine/graphene oxide/phenylalanine interface on adhesion and proliferation of multilineage-differentiating stress enduring cells

Zhou Jingjing1, 2, Sun Yixin1, 2, Sheng Yang1, 2, Zhang Rong1, 2   

  1. 1School of Materials Science and Engineering, Changzhou University, Changzhou 213614, Jiangsu Province, China; 2Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou University, Changzhou 213164, Jiangsu Province, China

  • Received:2022-04-02 Accepted:2022-06-06 Online:2023-04-08 Published:2022-09-08

  • Contact: Sun Yixin, MD, Associate professor, School of Materials Science and Engineering, Changzhou University, Changzhou 213614, Jiangsu Province, China; Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou University, Changzhou 213164, Jiangsu Province, China

  • About author:Zhou Jingjing, Master candidate, School of Materials Science and Engineering, Changzhou University, Changzhou 213614, Jiangsu Province, China; Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials, Changzhou University, Changzhou 213164, Jiangsu Province, China

Abstract: BACKGROUND: In recent years, multilineage-differentiating stress enduring (Muse) cell has attracted increasing attention due to its pluripotent differentiation capacity and non-tumorigenicity. Cell therapy based on Muse cells has shown exciting results in the animal experiments of many diseases. However, Muse cells face the difficulties of slow proliferation in long-term in vitro culture.  
OBJECTIVE: To investigate the effect of chirals interfaces on adhesion and proliferation of Muse cells.
METHODS: The polyethyleneimine/graphene oxide/phenylalanine chiral biointerfaces (PEI/GO/L-Phe and PEI/GO/D-Phe) were constructed based on the layer-by-layer self-assembly method using polyethyleneimine (PEI) as the substrate layer and graphene oxide (GO) and L/D-phenylalanine (L/D-Phe) sequentially deposited. The effect of self-assembled chiral biointerfaces on the adhesion and proliferation behavior of Muse cells was investigated.  
RESULTS AND CONCLUSION: (1) PEI/GO/L-Phe and PEI/GO/D-Phe chiral interfaces were successfully constructed based on facile self-assembly technology. (2) The statistical results showed that the chirality on the interface influenced Muse cell behaviors significantly. The initial adhesion and subsequent proliferation of Muse cells were enhanced by the PEI/GO/L-Phe interface, probably owing to the preferred adsorption of proteins, which further promote Muse cell proliferation.
Key words: regenerative medicine, Muse cells, chiral interfaces, layer self-assembly, phenylalanine enantiomers, adhesion, proliferation, protein adsorption


分享到:

Publishing Information

Publishing House of Chinese Journal of Tissue Engineering Research


The Official Publication of

Chinese Association of Rehabilitation Medicine

Contact Us

General editorial enquiries:

Email: bwb01@crter.org

Copyright related contact:

Email: crter@crter.org

Commercial Sales contact (Reprints, advertising, etc.):

Email: bwb@crter.org