2023, Vol. 27 ›› Issue (19): 3011-3016

Resveratrol promotes the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth

Yue Haiyun1, Sun Yinxue2, Bi Yingchun3   

Abstract: BACKGROUND: Resveratrol is a natural non-flavonoid polyphenol compound. Studies have shown that resveratrol has antioxidant, anti-inflammatory, anti-aging and anti-tumor activities, and promotes the osteogenic differentiation of bone marrow mesenchymal stem cells. However, whether resveratrol can promote the osteogenic differentiation of stem cells from human exfoliated deciduous teeth is even not expounded.  
OBJECTIVE: To investigate the effects of resveratrol on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth.
METHODS: Human stem cells from human exfoliated deciduous teeth were isolated and cultured by tissue block method. Osteogenic induced stem cells from human exfoliated deciduous teeth were treated with different concentrations of resveratrol. Cell activity was detected by CCK-8 assay. The number of mineralized nodular cells was detected by alizarin red staining. mRNA expression of osteogenic genes was detected by RT-qPCR and protein expression of Runt-related transcription factor 2 was detected by western blot assay.  
RESULTS AND CONCLUSION: (1) CCK-8 assay results showed that after 3 days of treatment, resveratrol at concentrations of (20, 40, 80, and 100 μmol/L) significantly inhibited the proliferation of stem cells from human exfoliated deciduous teeth. Therefore, 1, 5, and 10 μmol/L resveratrol could be used to treat stem cells from human exfoliated deciduous teeth in subsequent experiments to detect their effects on osteogenic differentiation. (2) With 10 μmol/L
resveratrol treatment, the number of osteogenic nodules of stem cells from human exfoliated deciduous teeth increased significantly compared with the control group. (3) Alkaline phosphatase, Runt-related transcription factor 2, and osteocalcin mRNA expression levels were increased after 10 μmol/L resveratrol treatment compared with the control group. (4) Runt-related transcription factor 2 protein expression levels of resveratrol treatment groups increased in a concentration-dependent manner compared with the control group. (5) The results indicate that appropriate concentration of resveratrol promotes the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth.

Key words: stem cell, mesenchymal stem cell, dental pulp stem cell, stem cells from human exfoliated deciduous teeth, resveratrol, osteogenesis, differentiation