2023, Vol. 27 ›› Issue (2): 252-257

Effects of extracorporeal shock wave on the proliferation and autophagy of chondrocytes from osteoarthritis rats

Wang Huanhuan1, Wang Qing2, Tang Peng3, Zhang Rui3, Min Hongwei1, 3   

Abstract: BACKGROUND: Extracorporeal shock wave is a new treatment method that has been widely used in various medical fields and plays an important role in the occurrence and development of osteoarthritis.
OBJECTIVE: To investigate the effects of extracorporeal shock wave on the proliferation and autophagy of chondrocytes from osteoarthritis rats in vitro and to explore the mechanism of extracorporeal shock wave in the treatment of osteoarthritis.
METHODS: Primary chondrocytes were isolated and cultured from the articular cartilage of extremities of 1-week-old clean-grade Sprague-Dawley rats. Chondrocytes at passage 3 were randomly divided into control group (without any treatment), interleukin-1β group (10 μg/L interleukin-1β for 24 hours), extracorporeal shock wave group (1.5×105 Pa extracorporeal shock wave for 500 times), interleukin-1β+extracorporeal shock wave group (10 μg/L interleukin-1β for 24 hours+1.5×105 Pa extracorporeal shock wave for 500 times). The viability and proliferation of chondrocytes were detected by cell counting kit-8 method. The mRNA expressions of Beclin 1, P62, Atg5 and LC3 were detected by real-time fluorescence quantitative PCR.
RESULTS AND CONCLUSION: Stimulation with interleukin-1β for 24 hours decreased the viability and proliferation of chondrocytes compared with the control group (P < 0.05). Compared with the interleukin-1β group, chondrocyte viability and proliferation were increased in the interleukin-1β+extracorporeal shock wave group (P < 0.05). The expression of Beclin 1, Atg5 and LC3 was down-regulated and P62 expression was increased in the interleukin-1β group compared with the control group (P < 0.05), whilst the expression of Beclin 1, Atg5 and LC3 was significantly increased and the expression of P62 was decreased in the interleukin-1β+extracorporeal shock wave group compared with the interleukin-1β group (P < 0.05). These results suggest that extracorporeal shock wave can promote the proliferation and autophagy of osteoarthritic chondrocytes, thus regulating the progression of osteoarthritis.
Key words: extracorporeal shock wave, osteoarthritis, chondrocyte, proliferation, autophagy, rat