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2023, Vol. 27 ›› Issue (20): 3143-3150

Screening and validation of neurogenic bladder miRNA-mRNA regulatory network

Guo Shuhui1, Yang Ye1, Jiang Yangyang2, Xu Jianwen1   

  1. 1The First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi Zhuang Autonomous Region, China; 2The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China

  • Received:2022-07-09 Accepted:2022-08-27 Online:2023-07-18 Published:2022-11-21

  • Contact: Xu Jianwen, MD, Chief physician, Professor, Doctoral supervisor, The First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi Zhuang Autonomous Region, China

  • About author:Guo Shuhui, Master candidate, Physician, The First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi Zhuang Autonomous Region, China

  • Supported by:

    the National Natural Science Foundation of China, No. 81960417 (to XJW); Guangxi Natural Science Foundation Project, No. 2018GXNSFAA050033 (to XJW)


Abstract: BACKGROUND: The miRNA-mRNA regulatory network plays an important role in various biological processes, but its specific mechanism in neurogenic bladder after spinal cord injury remains unclear.
OBJECTIVE: To mine the related targets of neurogenic bladder based on bioinformatics and to construct the miRNA-mRNA regulatory network, verified by animal experiments.
METHODS: The differential genes of neurogenic bladder were screened from GEO database to construct the miRNA-mRNA regulatory network. The differential genes were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses using the David database. Protein-protein interaction analysis was performed using the STRING database. Key genes were identified by Cytoscape and ROC curves. Finally the key genes were validated by RT-PCR in a rat model of neurogenic bladder.
RESULTS AND CONCLUSION: A total of 188 differential genes related to neurogenic bladder were identified. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that they were enriched mainly in inflammatory response, cytokine-cytokine receptor interaction, and chemokine signaling pathway. Two key regulatory networks, miR-147-brain-derived neurotrophic factor and miR-362-5p-Scn9a, were identified based on the Cytoscape and ROC curve results. RT-PCR results showed that miR-147, miR-362-5p, and brain-derived neurotrophic factor were highly expressed in the neurogenic bladder rats, while Scn9a was lowly expressed compared with the control rats. Overall, these findings indicate that miR-147-brain-derived neurotrophic factor and miR-362-5p-Scn9a actas important regulators of pathophysiological processes such as inflammation and oxidative stress, which are expected to become new targets for the diagnosis and treatment of neurogenic bladder.  

Key words: neurogenic bladder, spinal cord injury, bioinformatics, GEO database, differential genes, regulatory network, miRNA-mRNA, inflammation, oxidative stress


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Chinese Association of Rehabilitation Medicine

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