2023, Vol. 27 ›› Issue (6): 821-826

Protection of manganese oxide nanoparticles for bone marrow mesenchymal stem cell spreading against oxidative stress

Tian Qinyu1, 2, Tian Xinggui3, Tian Zhuang4, Sui Xiang2, Liu Shuyun2, Lu Xiaobo1, Guo Quanyi1, 2   

Abstract: BACKGROUND: During stem cell transplantation, the generation of a large number of free radicals damages the cells in situ, and then reducing the cell survival. In the past decade, the emergence of nanozymes provides a new method for scavenging excess free radicals. Among them, manganese oxide (Mn3O4) is one of the promising nanomaterials, because it could provide Mn trace elements in human body as well as its well antioxidative stress properties and biodegradability. Mn3O4 can be used as a new type of nanomaterial to protect the extension function of stem cells.
OBJECTIVE: To produce Mn3O4 nanoparticles for detecting its effect on scavenging and spreading function of bone marrow mesenchymal stem cells during oxidative stress.
METHODS:   Mn3O4 nanoparticles were prepared by hydrothermal method. Scanning electron microscope and X-ray diffraction were used to characterize the morphology and structure of Mn3O4, respectively. The particle size and zetapotential data of the nanoparticle in human-simulated environment were detected by the dynamic light scattering. The antioxidant capacity of Mn3O4 was tested in neutral environment. The CCK-8 and live-dead staining assays were utilized to verify the biological toxicity of Mn3O4 to bone marrow mesenchymal stem cells. H2O2 was used to induce oxidative stress of bone marrow mesenchymal stem cells. The effect of Mn3O4 on antioxidant ability and spreading ability of bone marrow mesenchymal stem cells was detected under oxidative stress.
RESULTS AND CONCLUSION: (1) The results of scanning electron microscope and X-ray diffraction exhibited that the material was Mn3O4 with average particle size about 70-80 nm. The dynamic light scattering showed that the particle size of the nanoparticles in PBS solution was about 100 nm, and the Zeta potential was about +20 mV. Antioxidant performance experiments showed that Mn3O4 had a certain antioxidant capacity. (2) The CCK-8 assay, live-death staining and reactive oxygen scavenging experiment demonstrated that Mn3O4 did not show biotoxicity and exhibited a certain level of reactive oxygen scavenging ability under 40 mg/L. (3) The cell spreading analysis showed that H2O2 had a significant inhibitory effect on the spreading function of bone marrow mesenchymal stem cells. After adding Mn3O4 into the cell culture microenvironment, the cell spreading area had no significant difference compared with the control group (P > 0.05). Moreover, the single use of Mn3O4 did not inhibit the spreading function of bone marrow mesenchymal stem cells (P > 0.05). (4) The results have shown that the addition of Mn3O4 nanoparticles in the cell microenvironment can resist oxidative stress and have a certain protective effect on the spreading function of bone marrow mesenchymal stem cells.
Key words: manganese oxide, nanoparticle, nanozyme, anti-oxidative stress, reactive oxygen species scavenging, stem cell transplantation, stem cell spreading, stem cell protection