2023, Vol. 27 ›› Issue (7): 1017-1022

Sustained releasing of pyrazinamide, capreomycin, moxifloxacin and amikacin loaded bone cement in vitro

Yuan Hucheng1, Ding Yongguo1, Ma Xuehua2, Ma Wenxin3, Sun Jianmin1, Wang Zili4, 5, Jin Weidong3, 5   

Abstract: BACKGROUND: Oral anti-tuberculosis drugs have serious systemic side effects after removal of bone and joint tuberculosis lesions. It is imperative to seek a sustained-release drug system that can not only save drug dosage, but also increase local drug concentration in lesions and reduce systemic blood drug concentration.
OBJECTIVE: To observe the sustained-release properties of polymethyl methacrylate bone cement loaded with antituberculosis drugs pyrazinamide, capreomycin, moxifloxacin, and amikacin in PBS artificial simulated body fluid.
METHODS: Polymethyl methacrylate bone cement Palacos R powder was mixed with antituberculosis drugs pyrazinamide, capreomycin, moxifloxacin, and amikacin in the proportion of 40 g:1.5 g and 40 g:2.5 g, respectively. The 20 mL of liquid monomer were added to prepare 8 groups of anti-tuberculosis drug-loaded bone cement standard specimens, with 5 samples in each group. In the control group, 40 g bone cement powder and 20 mL liquid monomer were mixed in the same way to prepare 5 standard bone cement samples without drugs. The drug was soaked in PBS and placed in 37 ℃ constant temperature water bath oscillator. The extract was taken at set time points. The drug concentration of each group was determined by high performance liquid chromatography.
RESULTS AND CONCLUSION: (1) The time of the lowest drug release concentration in PBS artificial simulated body fluid of pyrazinamide 1.5 g group, pyrazinamide 2.5 g group, capreomycin 1.5 g group, capreomycin 2.5 g group, moxifloxacin 1.5 g group, moxifloxacin 2.5 g group, amikacin 1.5 g group and amikacin 2.5 g group was 45, 60, 150, 150, 120, 120, 60 and 90 days, respectively. Among these groups, the capreomycin 1.5 g group, capreomycin 2.5 g group, moxifloxacin 1.5 g group, moxifloxacin 2.5 g group, and amikacin 2.5 g group had a longer drug release period, while the control group had no drug release. (2) It is concluded that polymethyl methacrylate bone cement loaded with capreomycin (1.5 g and 2.5 g), moxifloxacin (1.5 g and 2.5 g), and amikacin (2.5 g) has a long release cycle and good release performance.
Key words: polymethylmethacrylate, bone cement, antituberculosis drug, sustained release property, tuberculosis of bone and joint, focal debridement, high performance liquid chromatography, sustained-release material