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2024, Vol. 28 ›› Issue (33): 5320-5325

Mechanism by which miR-34a-5p/PLCD3 axis regulates osteoarthritis progression

Ying Pu, Xu Yue, Lu Tong, Xue Yi, Miao Yiming   

  1. Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, Jiangsu Province, China

  • Received:2023-08-14 Accepted:2023-10-12 Online:2024-11-28 Published:2024-01-30

  • Contact: Miao Yiming, Chief physician, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, Jiangsu Province, China

  • About author:Ying Pu, MD, Associate chief physician, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, Jiangsu Province, China

  • Supported by:

    Suzhou Science and Technology Development Plan Guiding Project, No. SKJYD2021007 (to YP); Suzhou Science and Technology Development Plan Guiding Project, No. SKJYD2021202 (to MYM); Suzhou “Science, Education and Health” Youth Science and Technology Project, No. KJXW2020068 (to YP); Key Project of Changshu Municipal Health Commission Science and Technology Plan, No. CSWS202118 (to YP); a grant from Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, No. cszyy201910 (to YP)


Abstract: BACKGROUND: Molecular mechanisms targeting the miRNA/mRNA axis to regulate osteoarthritis disease process have been studied. We identified the mRNA: phospholipase C delta 3 (PLCD3) and its target miRNA(miR-34a-5p) with clinical predictive value through previous bioinformatics studies, while experiments to verify their specific roles and mechanisms in regulating osteoarthritis are still lacking.
OBJECTIVE: To investigate the regulatory role and mechanism of miR-34a-5p/PLCD3 axis on osteoarthritis progression.
METHODS: The synovium of 15 patients with knee osteoarthritis was selected as the osteoarthritis group, and the synovium of 15 young patients with internal fixation of patellar fracture caused by trauma during the same period was selected as the control group. The expression of PLCD3 and miR-34a-5p in the synovium was detected by real-time PCR. Human fibroblast like synovial cells-osteoarthritis (HFLS-OA) cells were treated by cell transfection and divided into miR-34a-5p mimic group, pCDH-PLCD3 group, miR-34a-5p mimic+pCDH-PLCD3 group, miR-34a-5p inhibitor group, si-PLCD3 group, and miR-34a-5p inhibitor+si-PLCD3 group. The relationship between PLCD3 and miR-34a-5p expression was detected by real-time PCR. The effects of HFLS-OA cell viability and cell migration in each group were detected by CCK-8 assay and cell scratch test. Western blot assay was used to detect the expression level of apoptosis marker protein. The expression of inflammatory factors was detected by ELISA.
RESULTS AND CONCLUSION: (1) PLCD3 was a direct target of miR-34a-5p, and the expression levels of PLCD3 and miR-34a-5p were negatively correlated. (2) Upregulation of PLCD3 promoted proliferation of HFLS-OA cells and inhibited cell migration. The up-regulation of miR-34a-5p significantly inhibited the activity of HFLS-OA cells and enhanced cell migration. Overexpression of miR-34a-5p significantly increased the levels of Casp3 and Casp9 proteins in HFLS-OA cells, while overexpression of PLCD3 showed the opposite trend. (3) PLCD3 overexpression significantly increased the expression of interleukin 6 and tumor necrosis factor alpha in HFLS-OA cells, while miR-34a-5p mimics showed protective activity. (4) The miR-34a-5p/PLCD3 axis may affect the progression of osteoarthritis by regulating the inflammatory process or apoptosis of synovial cells.

Key words: osteoarthritis, synovial membrane, miR-34a-5p, phospholipase Cδ3, PLCD3, interleukin 6, tumor necrosis factor alpha


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