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2025, Vol. 29 ›› Issue (36): 7856-7862Academic progress and clinical application of in vitro synthetic microenvironment to promote maturation of human pluripotent stem cell-derived cardiomyocytesLiu Lu1, Zhong Chang2, Yu Xin2, Ren Chenyuan2, Gong Yangyang2, Zhou Ping2, Wang Yingbin1
Abstract: BACKGROUND: Human pluripotent stem cell-derived cardiomyocytes offer an ideal cellular resource for studying heart diseases, conducting drug screening, developing in vitro heart models, and exploring potential cell therapies. However, human pluripotent stem cell-derived cardiomyocytes are characterized by immaturity with limited specific gene expression, low Ca2+ processing levels, and underdeveloped structural, metabolic, and electrophysiological features. These limitations significantly impede the application of human pluripotent stem cell-derived cardiomyocytes. Key words: in vitro synthetic microenvironment, human pluripotent stem cell, cardiomyocyte, cell maturation, physical method, biochemical method, three-dimensional culture method, clinical application, review |