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2025, Vol. 29 ›› Issue (36): 7856-7862

Academic progress and clinical application of in vitro synthetic microenvironment to promote maturation of human pluripotent stem cell-derived cardiomyocytes

Liu Lu1, Zhong Chang2, Yu Xin2, Ren Chenyuan2, Gong Yangyang2, Zhou Ping2, Wang Yingbin1   

  1. 1Second Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China; 2School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China

  • Received:2024-08-19 Accepted:2024-10-22 Online:2025-12-28 Published:2025-03-24

  • Contact: Wang Yingbin, MD, Chief physician, Second Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China; Co-corresponding author: Zhou Ping, MD, Chief physician, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China

  • About author:Liu Lu, Master candidate, Second Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China. Zhong Chang, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China. Liu Lu and Zhong Chang contributed equally to this article.

  • Supported by:

    Natural Science Foundation of Gansu Province, No. 24JRRA1106 (to WYB)


Abstract: BACKGROUND: Human pluripotent stem cell-derived cardiomyocytes offer an ideal cellular resource for studying heart diseases, conducting drug screening, developing in vitro heart models, and exploring potential cell therapies. However, human pluripotent stem cell-derived cardiomyocytes are characterized by immaturity with limited specific gene expression, low Ca2+ processing levels, and underdeveloped structural, metabolic, and electrophysiological features. These limitations significantly impede the application of human pluripotent stem cell-derived cardiomyocytes.
OBJECTIVE: To review the academic progress and clinical application of promoting the maturation of human pluripotent stem cell-derived cardiomyocytes by in vitro synthetic microenvironment.
METHODS: CNKI, WanFang, VIP, PubMed, Web of Science, and Medline databases were searched, with “human pluripotent stem cells, human myocardial cells, hPSC-CMs, mature, OA, human pluripotent stem cell-derived cardiomyocytes, hPSC-CMs” as English search terms and “human pluripotent stem cells, cardiomyocytes, mature, OA, hPSC-CMs” as Chinese search terms. All relevant literature published from January 2002 to July 2024 was retrieved and 82 articles were included in the review.
RESULTS AND CONCLUSION: (1) In recent years, in vitro synthetic microenvironments have attracted extensive attention due to their excellent intrinsic properties such as stiffness, plasticity, nanoscale morphology, and chemical functionality. (2) Human pluripotent stem cell-derived cardiomyocytes can be used as an effective platform for the treatment of cardiovascular diseases. (3) Mechanical stimulation, electrical stimulation, addition of biochemical molecules, and three-dimensional culture methods are effective methods to promote the maturation of human pluripotent stem cell-derived cardiomyocytes, which can further promote the clinical application of human pluripotent stem cell-derived cardiomyocytes.

Key words: in vitro synthetic microenvironment, human pluripotent stem cell, cardiomyocyte, cell maturation, physical method, biochemical method, three-dimensional culture method, clinical application, review


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Chinese Association of Rehabilitation Medicine

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