2022, Vol. 26 ›› Issue (23): 3768-3772

Differences of trabecular microstructure and bone metabolism in two types of kidney-deficiency ovariectomized rats

He Xingpeng1, Zheng Liqin1, Li Pengfei2, 3, Yue Guiyang1, Li Zhihong4, Wu Minhui1, Lin Ziling5, 6   

Abstract: BACKGROUND: At present, ovariectomized rats are a mature and reliable animal model of osteoporosis, and establishment and observational evaluation of rat models of two types of kidney deficiency have been relatively detailed. However, further exploration is needed on the rat models of osteoporosis combined with syndromes of kidney-yang deficiency and kidney-yin deficiency. The sympathetic-parasympathetic nerve function test is used as a specific index to judge the syndrome of yin-deficiency and yang-deficiency, but this indicator is relatively insufficient for evaluating and analyzing the skeletal system of the two types of kidney-deficiency syndromes. “Integration of disease and syndrome” requires more experimental evidence and observational indicators.
OBJECTIVE: To compare trabecular microstructure parameters and serum bone metabolism indicators between osteoporosis rat models of kidney-yang deficiency and kidney-yin deficiency.
METHODS: The ovaries of 20 female Spraque-Dawley rats were surgically removed to make postmenopausal osteoporotic rat models. Ten weeks after operation, all model rats were randomly divided into two groups (n=10 per group): kidney-yang deficiency group and kidney-yin deficiency group. Gluteal intramuscular injection of hydrocortisone and intragastric administration of thyroid hormone tablets were given to the kidney-yang deficiency group and the kidney-yin deficiency group, respectively, once a day for 14 consecutive days. After modeling, micro-CT was used to detect trabecular microstructure parameters in the region of interest of the left femur, and ELISA was used to detect serum bone formation and bone resorption indicators.
RESULTS AND CONCLUSION: Two types of kidney-deficiency osteoporosis rat models were successfully established. Compared with the kidney-yang deficiency group, bone mineral density, bone volume/total volume, and trabecular number were significantly increased, trabecular thickness decreased, and serum bone formation and bone resorption indicators significantly increased in the kidney-yin deficiency group. Therefore, bone metabolism was faster in the kidney-yin deficiency group than the kidney-yang deficiency group. All these findings indicate significant differences in trabecular microstructure parameters and bone metabolism indicators between two kidney-yang and kidney-yin deficiency groups, and the mechanisms need to be further studied.
Key words: kidney-deficiency syndrome, ovariectomized rat, osteoporosis, Micro-CT, bone metabolism indicator